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ISOLATION OF A TAPETUM-SPECIFIC PROMOTER AND CONSTRUCTION OF A BINARY VECTOR OF RICE
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1006-687X.2005.04.002
- Key Word:
- 绒毡层;启动子;Touchdown PCR;克隆;载体构建
Abstract： 从籼稻川75A (Oryza sativa L.)黄化苗叶片中直接提取基因组总DNA. 根据文献报道的水稻花药绒毡层特异启动子Osg6B序列设计引物,采用Touchdown PCR 技术获得水稻花药绒毡层特异表达的启动子Tsp1,将该片段克隆在载体pMD18-T Vector上转化到大肠杆菌JM109,并酶切和PCR验证. 序列分析发现,该片段与Osg6B启动子同源性为96%,且具有真核生物启动子的多种特征序列,表明启动子Tsp1为来源于籼稻川75A中的水稻花药绒毡层特异表达的启动子. 利用该启动子对本实验室保存的质粒pIB467和pIB009进行改造,构建了一个可产生新型雄性不育系的双元植物表达载体pJH401. 图7参13
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