You Position: Home > Paper
PCR-DETECTION AND AS-PCR MARKER OF HIGH MOLECULAR WEIGHT GLUTENIN SUBUNITS IN COMMON WHEAT
( views:153, downloads:4 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 1
- DOI:
- 10.3321/j.issn:1006-687X.2005.01.005
- Key Word:
- 小麦;HMW-GS;Dx5基因;Dy10基因;PCR;AS-PCR
Abstract： 选用高分子量麦谷蛋白亚基(HMW-GS)在Glu-D1位点已知的26个普通小麦品种,包括15个(Triticum aestivum L.)川育系列品种及其11个亲本,根据其亚基 Dx5和Dy10基因的特异序列设计引物.通过对Dx5基因的PCR扩增及对Dy10基因的AS-PCR扩增,结果表明,所设计的引物对Dx5和Dy10都能扩增出特异条带,而携有Dx5基因的材料同时也携有Dy10基因.说明用所设计的引物可快速有效的鉴定出普通小麦中是否带有优质的5+10亚基. 图4 参15
- [1]茹岩岩,晏月明.小麦谷蛋白亚基基因的PCR鉴定及其在品质改良中的应用[J].2001,(2).
- [2]张晓科,魏益民,王新中,等.高分子量麦谷蛋白亚基基因Dx5的PCR检测[J].2003,(1).
- [3]周利,吴瑜,李竹林.川育小麦高分子量谷蛋白亚基组成分析[J].2004,(2).
- [4]U, Landegren,R, Kaiser,C T, Caskey,etc.DNA diagnostics--molecular techniques and automation.[J].1988,242.
- [5]Smith Rex L.,Schweder M. E.,Barnett R. D..Identification of Glutenin Alleles in Wheat and Triticale Using PCR-Generated DNA Markers[J].1994.
- [6]R.D'Ovidio,E.Porceddu,D.Lafiandra.PCR analysis of genes encoding allelic variants of high-molecular-weight glutenin subunits at theGlu-D1locus[J].1994,88(2).
- [7]Dong H.,Cox T. S.,Sears R. G.,etc.High Molecular Weight Glutenin Genes: Effects on Quality in Wheat[J].1991.
- [8]M A, Saghai-Maroof,K M, Soliman,R A, Jorgensen,etc.Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics.[J].1984,81.
- [9]R.D.Thompson,D.Bartels,N.P.Harberd.Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit[J].1985,13(19).
- [10]R.D'Ovidio,O.D.Anderson.PCR analysis to distinguish between alleles of a member of a multigene family correlated with wheat bread-making quality[J].1994,88(6).
- [11]T, Sugiyama,A, Rafalski,D, Peterson,etc.A wheat HMW glutenin subunit gene reveals a highly repeated structure.[J].1985,13.