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TRANSGENIC ELITE INDICA RESTORER LINE AND GENETIC STABILITY OF TRANSGENES

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
Issue:
5
DOI:
10.3321/j.issn:1006-687X.2004.05.002
Key Word:
籼稻;抗虫基因;遗传转化;雪花莲外源凝集素基因;遗传稳定性;indica rice;insect-resistant gene;genetic transformation;Galanthus nivalis agglutinin gene (gna);genetic stability

Abstract: This paper reports that the transgenic elite indica restorer line containing snowdrop lectin (Galanthus nivalis agglutinin, GNA) gene gna was obtained by biolistics and identified by PCR, PCR-Southern blotting and Southern blotting that the transgenes were integrated with estimated multi-copy at the same location of the genome of SH527 and transmitted stably from the first generation (T0) of transgenic plants to the third generation (T2). The plant height and seed-setting percentage of the first generation (T0) of transgenic plants were reduced apparently as compared with the corresponding plants derived from seeds and untransformed calli. However, this kind of variation could not be inherited stably. The protein analysis showed that the insect-resistant gene expressed in T0, T1 and T2 plants. The expressed protein had the biological activity and the ratio of GNA from transgenic plants to their total soluble protein was about 0.1%. The usage of genetic engineering in the breeding programs of hybrid rice was also discussed. Fig 4, Tab 2, Ref 18

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