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OBTAINING INSECT-RESISTANT TRANSGENIC SWEET PEPPER TRANSFORMED WITH MODIFIED CpTI GENE(sck)
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 3
- DOI:
- 10.3321/j.issn:1006-687X.2002.03.003
- Key Word:
- 甜椒;修饰的豇豆胰蛋白酶抑制剂基因;根癌农杆菌;茎伸长;抗虫
Abstract： 以12～14 d苗龄的甜椒带柄子叶为外植体,经根癌农杆菌(Agrobacteriumtumefaciens)介导,将修饰的豇豆胰蛋白酶抑制剂基因(sck)导入甜椒杂交种"中椒5号"和常规种"茄门"中,在对甜椒转化系统和芽丛诱导茎伸长的条件进行优化研究后,获得了卡那霉素抗性植株,最高转化频率为16.7%.卡那霉素抗性筛选、PCR检测和Southern blot杂交均证实,nptⅡ基因和sck基因已整合进甜椒基因组中.室内离体叶片饲虫和田间自然抗虫性鉴定进一步证明,转基因植株对棉铃虫(Heliothis armtgera Hubner)具有一定抗性.图4表2参13
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