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VESICLE FORMATION IN FUSION PROCESS OF SAINFOIN AND ALFALFA PROTOPLASTS
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1006-687X.2001.05.001
- Key Word:
- 原生质体融合;小泡;苜蓿;红豆草;PEG
Abstract： 通过高钙离子浓度、高pH值方法结合PEG处理诱导红豆草羟脯氨酸抗性系和苜蓿根癌农杆菌转化系原生质体融合.融合机制表现为两种形式,第一种为相连膜在中间部位形成一个大的小泡,第二种为随相连膜的解体产生很多较小的泡状结构.融合过程中形成的小泡直接来自凝集膜,而不是亚显微小泡相互融合的产物.温度明显影响原生质体融合的频率和速度.甘油和二甲基亚砜可以防止膜破裂,从而促进原生质体融合.培养20 d以后,在选择培养基得到含10～20个细胞的细胞团.图3参6
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