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INOCULATION，ISOLATION OF ENDOPHYTIC DIAZTOROPHIC BACTERIA AND THEIR COLONIZATION IN MAIZE ROOT TISSUE UNDER GNOTOBIOTIC CONDITION
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 3
- DOI:
- No doi available
- Key Word:
- 玉米;内生固氮菌;回接分离;绿色荧光蛋白基因;定殖
Abstract： 对从玉米茎内分离的18株固氮菌进行了回接分离试验，经多碳源无氮培养基、菌落形态、颜色与REP-PCR相结合的方法，筛选、鉴定出了两株具有较高乙炔还原活性和能在玉米体内定殖的菌株R1和R6.把携带gfp标记基因的质粒pKK223-GFP和nifH-gfp的质粒pMGFP2.1分别转化R1和R6，得到携带gfp，且菌落带绿色荧光的转化子R1A和R6A，以及携带nifH-gfp的转化子R1B和R6B.在限菌条件下对R1A和R6A在玉米根内的定殖以及R1B和R6B中nifH-gfp融合基因的表达进行了研究，结果表明了R1A和R6A主要定殖在根的皮层细胞、胞间隙、中柱细胞和导管内，有时在活的细胞内也可检测到.R1B和R6B中的融合基因因受玉米根内营养以及其它与固氮酶基因表达相关因素的限制，很难在玉米根内表达，只有在添加了1‰蔗糖后，nifH-gfp才能在根内表达. 图2 表2 参15
- [1]Eduardo Lima,Robert M. Boddey,Johanna Döbereiner.Quantification of biological nitrogen fixation associated with sugar cane using a 15N aided nitrogen balance[J].1987,19(2).
- [2]B, Reinhold-Hurek,T, Hurek.Life in grasses: diazotrophic endophytes.[J].1998,6(4).
- [3]VladimirA.Cavalcante,J.Dobereiner.A new acid-tolerant nitrogen-fixing bacterium associated with sugarcane[J].1988,108(1).
- [4]Triplett EW,Ludden PW,Palus JA,etc.A diazotrophic bacterial endophyte isolated from stems of Zea mays L. and Zea luxurians Iltis and Doebley.[J].1996,186(1).
- [5]F J, de Bruijn.Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria.[J].1992,58.