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CHEMICAL MODIFICATION OF TRYPTOPHAN RESIDUES AND STUDIES ON LECTIN FROM MILLETTIA DIELSIANA HARMS EX DIELS
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1006-687X.2000.04.005
- Key Word:
- 岩豆;凝集素;色氨酸残基;化学修饰;荧光光谱;糖蛋白
Abstract： 从岩豆(Millettia dielsiana Harms ex Diels)种子中分离纯化得到的岩豆凝集素(简称MDL)经链霉蛋白酶水解,测得其分子中含有4个色氨酸(Trp)残基.用N-溴代丁二酰亚胺(NBS)对 MDL分子中的色氨酸残基进行化学修饰,在无变性剂存在下有3.6个Trp残基被修饰,在变性条件下可修饰3.8个Trp残基.修饰后MDL活性均丧失,且甘露糖对MDL活性具有保护作用.表明Trp残基是MDL凝集活性所必需的基团,且位于MDL糖结合部位.同时采用荧光光谱实验发现,随着NBS的加入,MDL荧光强度不断减弱,但发射谱形状和最大发射波长无大的变化,提示Trp残基可能位于MDL分子表面的疏水区内.图3 参8
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