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Stability and accuracy of the identification of Notopterygii Rhizoma et Radix using the ITS/ITS2 barcodes

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Author:
No author available
Journal Title:
Acta Pharmaceutica Sinica
Issue:
8
DOI:
No doi available
Key Word:
羌活;ITS/ITS2;物种鉴定;稳定性;准确性

Abstract: 为验证DNA条形码鉴定的稳定性与准确性,本文选用羌活药材作为研究对象,对31份样本提取基因组DNA,通过聚合酶链式反应(polymerase chain reaction,PCR)扩增内部转录间隔区(internal transcribed spacer,ITS)序列并进行双向测序,所得序列经CodonCode Aligner拼接后,采用MEGA5.0软件与其混伪品进行序列比对,计算种内和种间距离,构建邻接树(neighbor-joining tree,NJ Tree).ITS2序列采用基于隐马尔可夫模型(hidden Markov model,HMMer)的注释方法获得.结果表明,羌活药材ITS序列长度为603~604 bp,ITS2序列长度均为228 bp,羌活药材ITS/ITS2序列单倍型与其基原植物叶片序列一致.两种基原植物ITS/ITS2序列种内平均kimura 2-parameter (K2P)遗传距离均远远小于其与混伪品的种间平均K2P遗传距离;NJ树结果显示羌活、宽叶羌活与其混伪品均可明显区分,表现出良好单系性.因此ITS/ITS2序列作为DNA条形码能稳定、准确鉴别羌活药材,为保障临床安全用药提供了新的技术手段.

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