Stability and accuracy of the identification of Notopterygii Rhizoma et Radix using the ITS/ITS2 barcodes

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XIN Tian-yi()
YAO Hui()
LUO Kun()
MA Xiao-chong()
HAN Jian-ping()
LIN Yu-lin()
SONG Jing-yuan()
CHEN Shi-lin()
Journal Title:
Acta Pharmaceutica Sinica
Volume 47, Issue 08, 2012
Key Word:


  • [1]Li DZ,Liu JQ,Chen ZD,et al.Plant DNA barcoding in China[J].J Syst Evol,2011,49:165-168.
  • [2]Chen SL,Yao H,Han JP,et al.Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species[J].PLoS One,2010,5:e8613.
  • [3]Yao H,Song JY,Liu C,et al.Use of ITS2 region as the universal DNA barcode for plants and animals[J].PLoS One,2010,5:el3102.
  • [4]Chinese Plant BOL Group.Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants[J].Proc Natl Acad Sci USA,2011,108:19641-19646.
  • [5]Chinese Pharmacopoeia Committee.Pharmacopoeia of the People's Republic of China(中华人民共和国药典)[S].Part Ⅰ.Beijing:China Medical Science Press,2010:170.
  • [6]The Chinese Academy of Sciences.Flora of China (中国植物志)[M].Vol 55.Beijing:Science Press,1979:188-191.
  • [7]Zhou Y,Jiang SY,Ma XJ,et al.Resource crisis and protective measures on Rhizoma et Radix Notopterygii[J].Chin Tradit Herb Drugs (中草药),2003,34:S12-14.
  • [8]Wang YP,Pu FD,Wang PL,et al.Study on the systematics of the Chinese endemic genus Notopterygium[J].Acta Bot Yunnan(云南植物研究),1996,18:424-430.
  • [9]Pu FD,Wang PL,Zheng ZH,et al.A reclassification of Notopterygium Boissieu (Umbelliferae)[J].Acta Phytotaxon Sin (植物分类学报),2000,38:430-436.
  • [10]Gao LH,Fang ZS.Integrative analysis and evaluation of wild Rhizoma et Radix Notopterygii resource in Gansu[J].Pratacult Sci(草业科学),2007,24:11-14.
  • [11]Sun H,Jiang SY,Zhou Y,et al.Present situation and ethnobotany survey of Chinese and Tibetan traditional medicinal plants of Notopterygium[J].World Sci-Tech R&D(世界科技研究与发展),2004,26:42-47.
  • [12]Wang S,Xie Y.China Species Red List(中国物种红色名录)[M].Part Ⅰ.Beijing:Higher Education Press,2004.
  • [13]Xu GJ,Xu LS,Wang ZT.Commonly Used Chinese Materia Medica Variety Systematization and Quality Research (South Cooperation Group)(常用中药材品种整理和质量研究(南方协作组))[M].Part Ⅲ.Fuzhou:Fujian Science and Technology Press,1997:190-227.
  • [14]Wu XM,Yuan WS.Identification of Notopterygium and the mixed supplies[J].Chin Health Innovat(中国医疗前沿),2007,1:113.
  • [15]Gu Q,Zhang YF.Identification of Rhizoma et Radix Notopterygii and its varieties[J].Lishizhen Med Mater Med Res (时珍国医国药),2001,12:947.
  • [16]Lin L,Li CY,Yang FD.Study on resources of genuine traditional Chinese drugs of Rhizoma et Radix Notopterygii in Gansu province[J].J Gansu Tradit Chin Med(甘肃中医学院学报),2002,19:58-63.
  • [17]Chen SL,Yao H,Song JY,et al.Use of DNA barcoding to identify Chinese medicinal materials[J].World Sci Technol Mod Tradit Chin Med(世界科学技术-中医药现代化),2007,9:7-12.
  • [18]Chen SL,Pang XH,Yao H,et al.Identification system and perspective for DNA barcoding traditional Chinese materia medica[J].World Sci Technol Mod Tradit Chin Med(世界科学技术-中医药现代化),2011,13:747-754.
  • [19]Chen SL.Molecular Identification of Chinese Materia Medica Using DNA Barcodes(中药DNA条形码分子鉴定)[M].Beijing:People's Medical Publishing House,2012:248-251.
  • [20]Gao T,Yao H,Song JY,et al.Identification of medicinal plants in the family Fabaceae using a potential DNA barcode ITS2[J].J Ethnopharmacol,2010,130:116-121.
  • [21]Pang XH,Song JY,Zhu YJ,et al.Applying plant DNA barcodes for Rosaceae species identification[J].Cladistics,2011,27:165-170.
  • [22]Gao T,Yao H,Song JY,et al.Evaluating the feasibility of using candidate DNA barcodes in discriminating species of the large Asteraceae family[J].BMC Evol Biol,2010,10:324.
  • [23]Pang XH,Song JY,Zhu YJ,et al.Using DNA barcoding to identify species within Euphorbiaceae[J].Planta Med,2010,76:1784-1786.
  • [24]Zhu YJ,Chen SL,Yao H,et al.DNA barcoding the medicinal plants of the genus Paris[J].Acta Pharm Sin (药学学报),2010,45:376-382.
  • [25]Sun ZY,Chen SL,Yao H,et al.Identification of Notopterygii Rhizoma et Radix and its adulterants using DNA barcoding method based on ITS2 sequence[J].Chin Tradit Herb Drugs (中草药),2012,43:568-571.
  • [26]Han JP,Li MN,Luo K,et al.Identification of Daturae Flos and its adulterants based on DNA barcoding technique[J].Acta Pharm Sin(药学学报),2011,46:1408-1412.
  • [27]Tamura K,Peterson D,Peterson N,et al.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Mol Biol Evol,2011,28:2731-2739.
  • [28]Keller A,Schleicher T,Schultz J,et al.5.8S-28S rRNA interaction and HMM-based ITS2 annotation[J].Gene,2009,430:50-57.
  • [29]Hollingsworth PM.Refining the DNA barcode for land plants[J].Proc Natl Acad Sci USA,2011,108:19451-19452.
  • [30]Coleman AW.Pan-eukaryote ITS2 homologies revealed by RNA secondary structure[J].Nucleic Acids Res,2007,35:3322-3329.
  • [31]Koch MA,Dobes C,Mitchell-Olds T.Multiple hybrid formation in natural populations:concerted evolution of the internal transcribed spacer of nuclear ribosomal DNA (ITS) in North American Arabis divaricarpa (Brassicaceae)[J].Mol Biol Evol,2003,20:338-350.
  • [32]Chen SL,Xu J,Liu C,et al.Genome sequence of the model medicinal mushroom Ganoderma lucidum[J].Nat Commun,2012,3:913.
  • [33]Kress WJ,Wurdack KJ,Zimmer EA,et al.Use of DNA barcodes to identify flowering plants[J].Proc Natl Acad Sci USA,2005,102:8369-8374.
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