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Cloning and analysis of squalene synthase (HsSQS1) gene in Huperzia serrata

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Author:: No author available

Journal Title:: Acta Pharmaceutica Sinica

Issue:: 8

DOI:: No doi available

Key Word:: 蛇足石杉;鲨烯合酶;三萜合成

Abstract：鲨烯合酶(squalene synthase,SQS)是植物萜类化合物生物合成途径的关键酶.本研究根据课题组已获得的蛇足石杉(Huperzia serrata)转录组数据中的SQS1转录本序列,设计基因全长扩增引物,利用RT-PCR方法获得蛇足石杉SQS1基因的全长cDNA序列；利用实时荧光定量PCR方法检测了HsSQS1基因在蛇足石杉根、茎、叶中的表达情况；对HsSQS1基因进行生物信息学分析,并预测HsSQS1编码蛋白的结构与功能.研究结果表明,克隆获得的蛇足石杉SQS1基因长为1263 bp,编码420个氨基酸,命名为HsSQS1,GenBank登录号JQ004938.HsSQS1基因在蛇足石杉的根中表达丰度高于茎和叶.本研究成功克隆并分析了蛇足石杉SQS1的全长序列,为进一步阐明蛇足石杉三萜代谢途径奠定基础.

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