Research progress on intracellular metabolites based on metabolomics

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ZHANG Chen-chen()
SUN Li-xin()
Journal Title:
Acta Pharmaceutica Sinica
Volume 47, Issue 08, 2012
Key Word:


  • [1]Nicholson JK,Connelly J,Lindon JC,et al.Metabonomics:a platform for studying drug toxicity and gene function[J].Nat Rev Drug Discov,2002,1:153-161.
  • [2]Nicholson JK,Lindon JC,Holmes E.'Metabonomics':understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J].Xenobiotica,1999,29:1181-1189.
  • [3]Sumner LW,Mendes P,Dixon RA.Plant metabolomics:large-scale phytochemistry in the functional genomics era[J].Phytochemistry,2003,62:817-836.
  • [4]Fiehn O.Metabolomics-the link between genotypes and phenotypes[J].Plant Mol Biol,2002,48:155-171.
  • [5]Kell DB.Metabolomics and systems biology:making sense of the soup[J].Curr Opin Microbiol,2004,7:296-307.
  • [6]Villas-B(o)as SG,Nielsen J,Smedsqaard J,et al.Metabolome Analysis:An Introduction[M].New Jersey:Wiley Press,2007.
  • [7]Dettmer K,Numberger N,Kaspar H,et al.Metabolite extraction from adherently growing mammalian cells for metabolomics studies:optimization of harvesting and extraction protocols[J].Anal Bioanal Chem,2011,399:1127-1139.
  • [8]Lorenz MA,Burant CF,Kennedy RT.Reducing time and increasing sensitivity in sample preparation for adherent mammalian cell metabolomics[J].Anal Chem,2011,83:3406-3414.
  • [9]Teng Q,Huang W,Collette TW,et al.A direct cell quenching method for cell-culture based metabolomics[J].Metabolomics,2009,5:199-208.
  • [10]Sellick CA,Hansen R,Maqsood AR,et al.Effective quenching processes for physiologically valid metabolite profiling of suspension cultured mammalian cells[J].Anal Chem,2009,81:174-183.
  • [11]Weibel KE,Mor JR,Fiechter A.Rapid sampling of yeast cells and automated assays of adenylate,citrate,pyruvate and glucose-6-phosphate pools[J].Anal Biochem,1974,58:208-216.
  • [12]Volmer M,Northoff S,Scholz S,et al.Fast filtration for metabolome sampling of suspended animal cells[J].Biotechnol Lett,2011,33:495-502.
  • [13]Sellick CA,Knight D,Croxford AS,et al.Evaluation of extraction processes for intracellular metabolite profiling of mammalian cells:matching extraction approaches to cell type and metabolite targets[J].Metabolomics,2010,6:427-438.
  • [14](A)lvarez-Sánchez B,Priego-Capote F,Luque de Castro MD.Metabolomics analysis Ⅱ.Preparation of biological samples prior to detection[J].Trends Anal Chem,2010,29:120-127.
  • [15]Ritter JB,Genzel Y,Reichl U.Simultaneous extraction of several metabolites of energy metabolism and related substances in mammalian cells:optimization using experimental design[J].Anal Biochem,2008,373:349-369.
  • [16]Bennett BD,Yuan J,Kimball HE,et al.Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach[J].Nat Protoc,2008,3:1299-1311.
  • [17]Hofmann U,Maier K,Niebel A,et al.Identification of metabolic fluxes in hepatic cells from transient 13C-labeling experiments:part Ⅰ.Experimental observations[J].Biotechnol Bioeng,2008,100:344-354.
  • [18]Bullinger D,Neubauer H,Fehm T,et al.Metabolic signature of breast cancer cell line MCF-7:profiling of modified nucleosides via LC-IT MS coupling[J].BMC Biochem,2007,8:25-38.
  • [19]Liebich HM,Muller-Hagedorn S,Klaus F,et al.Chromatographic,capillary electrophoretic and matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis of urinary modified nucleosides as tumor markers[J].J Chromatogr A,2005,1071:271-275.
  • [20]Huang D,Zhang YZ,Chen XG.Analysis of intracellular nucleoside triphosphate levels in normal and tumor cell lines by high-performance liquid chromatography[J].J Chromatogr B,2003,784:101-109.
  • [21]Jia J,Zhang H,Zhao L,et al.An optimized ion-pair HPLC method for simultaneous analysis of nucleoside triphosphate levels in hepatoma cell line[J].Chromatographia,2011,73:755-759.
  • [22]Lehtimaki KK,Valonen PK,Griffin JL,et al.Metabolite changes in BT4C rat gliomas undergoing ganciclovir-thymidine kinase gene therapy-induced programmed cell death as studied by 1H NMR spectroscopy in vivo,ex vivo,and in vitro[J].Biol Chem,2003,278:45915-45923.
  • [23]Williams SN,Anthony ML,Brindle KM.Induction of apoptosis in two mammalian cell lines results in increased levels of fructose-1,6-bisphosphate and CDP-choline as determined by 31p MRS[J].Magn Reson Med,1998,40:411-420.
  • [24]Anthony ML,Zhao M,Brindle KM.Inhibition of phosphatidylcholine biosynthesis following induction of apoptosis in HL-60 cells[J].Biol Chem,1999,274:19686-19692.
  • [25]Wang J,Lin T,Cai ZW,et al.Analysis of adenosine phosphates in HepG-2 cell by a HPLC-ESI-MS system with porous graphitic carbon as stationary phase[J].J Chromatogr B,2009,877:2019-2024.
  • [26]Lu W,Qian SC,Wang FM,et al.Metabolism of amino acid in human liver cancer cell line in vitro[J].Tianjin Med J(天津医药),2002,30:643-645.
  • [27]Kao YY,Liu KT,Huang MF,et al.Analysis of amino acids and biogenic amines in breast cancer cells by capillary electrophoresis using polymer solutions containing sodium dodecyl sulfate[J].J Chromatogr A,2010,1217:582-587.
  • [28]Ippolito JE,Merritt ME,B(a)ickhed F,et al.Linkage between cellular communications,energy utilization,and proliferation in metastatic neuroendocrine cancers[J].Proc Natl Acad Sci USA,2006,103:12505-12510.
  • [29]Griffin JL,Lehtim(a)ki KK,Valonen PK,et al.Assignment of 1H nuclear magnetic resonance visible polyunsaturated fatty acids in BT4C gliomas under going ganciclovir-thymidine kinase gene therapy-induced programmed cell death[J].Cancer Res,2003,63:3195-3201.
  • [30]Warburg O.On the origin of cancer cells[J].Science,1956,123:309-314.
  • [31]Yang C,Richardson AD,Osterman A,et al.Profiling of central metabolism in human cancer cells by two-dimensional NMR,GC-MS analysis,and isotopomer modeling[J].Metabolomics,2008,4:13-29.
  • [32]Centelles JJ,Montoya AR,Lim S,et al.Metabolic profile and quantification of deoxyribose synthesis pathways in HepG-2 cells[J].Metabolomics,2007,3:105-111.
  • [33]Lu SB,Sun XG,Shi CO,et al.Determination of tricarboxylic acid cycle acids and other related substances in cultured mammalian cells by gradient ion-exchange chromatography with suppressed conductivity detection[J].J Chromatogr A,2003,1012:161-168.
  • [34]Maddula S,Baumbach JI.Heterogeneity in tumor cell energetic metabolome at different cell cycle phases of human colon cancer cell lines[J].Metabolomics,2011,7:509-523.
  • [35]Guo B,Dai RK.Current trends in analytical methodologies and experimental strategies for metabonomics[J].Chin J Health Lab Technol (中国卫生检验杂志),2007,17:554-563.
  • [36]Bugrim A,Nikolskaya T,Nikolsky Y.Early prediction of drug metabolism and toxicity:systems biology approach and modeling[J].Drug Discov Today,2004,9:127-135.
  • [37]Li H,Yu ZG,Zu XY,et al.Classification of the cell lines in the extraction of intracellular metabolites based on ultra performance liquid chromatography-time of flight mass spectrometry[J].Chin J Chromatogr(色谱),2009,27:387-390.
  • [38]Weljie AM,Bondareva A,Zang P,et al.1H NMR metabolomics identification of markers of hypoxia-induced metabolic shifts in a breast cancer model system[J].J Biomol NMR,2011,49:185-193.
  • [39]Shi DY,Liu SL,Li HR,et al.Regulation of metabolism signaling in heptoma cell by hypoxic stress[J].Prog Biochem Biophys(生物化学与生物物理进展),2006,33:869-876.
  • [40]Lu SB,Sun XM,Zhang YX,et al.Insight into metabolism of CHO cells at low glucose concentration on the basis of the determination of intracellular metabolites[J].Process Biochem,2005,40:1917-1921.
  • [41]Hartmann M,Zimmermann D,Nolte J.Changes of the metabolism of the colon cancer cell line SW-480 under serum-free and serum-reduced growth conditions[J].In Vitro Cell Dev Biol Anim,2008,44:458-463.
  • [42]Griffin JL,Williams HJ,Sang E,et al.Metabolic profiling of genetic disorders:a muhitissue 1H nuclear magnetic resonance spectroscopic and pattern recognition study into dystrophic tissue[J].Anal Biochem,2001,293:16-21.
  • [43]Carli D,Honorat M,Cohen S,et al.Simultaneous quantification of 5-FU,5-FUrd,5-FdUrd,5-FdUMP,dUMP and TMP in cultured cell models by LC-MS/MS[J].J Chromatogr B,2009,877:2937-2944.
  • [44]Cohen S,Megherbi M,Jordheim PL,et al.Simultaneous analysis of eight nucleoside triphosphates in cell lines by liquid chromatography coupled with tandem mass spectrometry[J].J Chromatogr B,2009,877:3831-3840.
  • [45]Van Moorsel CJA,Bergman AM,Veerman G,et al.Differential effects of gemcitabine on ribonucleotide pools of twenty-one solid tumour and leukaemia cell lines[J].Biochim Biophys Acta,2000,1474:5-12.
  • [46]Chen P,Liu ZF,Liu S J,et al.A LC-MS/MS method for the analysis of intracellular nucleoside triphosphate levels[J].Pharm Res,2009,26:1504-1515.
  • [47]Zhang W,Tan SL,Paintsil E,et al.Analysis of deoxyribonucleotide pools in human cancer cell lines using a liquid chromatography coupled with tandem mass spectrometry technique[J].Biochem Pharmacol,2011,82:411-417.
  • [48]Van Rompay AR,Jonansson M,Karlsson A.Phosphorylation of nucleosides and nucleoside analogs by mammalian nucleoside monophosphate kinases[J].Pharmacol Ther,2000,87:189-198.
  • [49]Pan X,Wilson M,Mirbahai L,et al.In vitro metabonomic study detects increases in UDP-GlcNAc and UDP-GalNAc,as early phase markers of cisplatin treatment response in brain tumor cells[J].J Proteome Res,2011,10:3493-3500.
  • [50]Bayet-Robert M,Lim S,Barthomeuf C,et al.Biochemical disorders induced by cytotoxic marine natural products in breast cancer cells as revealed by proton NMR spectroscopy-based metabolomics[J].Biochem Pharmacol,2010,80:1170-1179
  • [51]Griffin JL,Pole JC,Nicholson JK,et al.Cellular environment of metabolites and a metabonomic study of tamoxifen in endometrial cells using gradient high resolution magic angle spinning 1H NMR spectroscopy[J].Biochim Biophys Acta,2003,1619:151-158.
  • [52]Cong LN,Liu Q,Li XZ,et al.Metabolic footprinting and its application in metabolomics[J].J Liaoning Norm Univ (Nat Sci Ed)(辽宁师范大学学报自然科学版),2006,4:477-479.
  • [53]Kell DB,Brown M,Davey HM,et al.Metabolic footprinting and systems biology:the medium is the message[J].Nat Rev Microbiol,2005,3:557-565
  • [54]Nielsen J,Oliver S.The next wave in metabolome analysis[J].Trends Biotech,2005,23:544-546.
  • [55]Buchholz A,Hurlebaus J,Wandrey C,et al.Metabolomics:quantification of intracellular metabolite dynamics[J].Biomol Eng,2002,19:5-15.
  • [56]Villas-Boas SG,Noel S,Lane GA,et al.Extracellular metabolomics:a metabolic footprinting approach to assess Wber degradation in complex media[J].Anal Biochem,2006,349:297-305.
  • [57]Zang L,Frenkel R,Simeone J,et al.Metabolomics profiling of cell culture media leading to the identification of riboflavin photosensitized degradation of tryptophan causing slow growth in cell culture[J].Anal Chem,2011,83:5422-5430.
  • [58]Pandher R,Ducruix C,Eccles SA,et al.Cross-platform Q-TOF validation of global exo-metabolomic analysis:application to human glioblastoma cells treated with the standard PI 3-Kinase inhibitor LY294002[J].J Chromatogr B,2009,877:1352-1358.
  • [59]Miccheli AT,Miccheli A,Clemente RC,et al.NMR-based metabolic profiling of human hepatoma cells in relation to cell growth by culture media analysis[J].Biochim Biophys Acta,2006,1760:1723-1731.
  • [60]Pasikanti KK,Norasmara J,Cai SR,et al.Metabolic footprinting of tumorigenic and nontumorigenic uroepithelial cells using two-dimensional gas chromatography time-of-flight mass spectrometry[J].Anal Bioanal Chem,2010,398:1285-1293.
  • [61]Allen J,Davey HM,Broadhurst D,et al.High-throughput classification of yeast mutants for functional genomics using metabolic footprinting[J].Nat Biotechnol,2003,21:692-696.
  • [62]Dowlatabadi R,Weljie AM,Thorpe TA,et al.Metabolic footprinting study of white spruce somatic embryogenesis using NMR spectroscopy[J].Plant Physiol Biochem,2009,47:343-350.
  • [63]Li J,Wu XJ,Liu CX,et al.Application of new method for data processing in metabonomic studies[J].Acta Pharm Sin (药学学报),2006,41:47-53.
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