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Screening based on response surface methodology of multi-fractions traditional Chinese medicine with anti-influenza virus neuraminidase activity:take Shuanghuanglian injection as an example
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- Author:
- No author available
- Journal Title:
- Acta Pharmaceutica Sinica
- Issue:
- 4
- DOI:
- No doi available
- Key Word:
- 组分中药;筛选模式;响应面设计;流感病毒神经氨酸酶活性抑制;双黄连注射液
Abstract： 旨在建立组分中药组方筛选新方法,以期客观评估多组分内在关系及优化配比.以流感病毒神经氨酸酶(NA)活性抑制率为指标,采用Box-Behnken响应曲面设计法,对双黄连注射液(SHL)中主要指标成分进行抑制NA活性筛选,探讨其活性组分间的相互作用关系,并以SHL为参照,预测组合成分的最佳配比.结果表明,原方中绿原酸、隐绿原酸、咖啡酸和黄芩苷有较强的NA活性抑制作用,且绿原酸与隐绿原酸间存在拮抗作用,咖啡酸对其他组分具有协同增效作用；连翘酯苷A、连翘苷等未见明显(或协同)NA活性抑制作用；拟合的模型预测组分最佳配比为绿原酸-隐绿原酸-咖啡酸-黄芩苷(107 μg·mL-1∶279 μg·mL-1∶7.99 μg·mL-1∶92 μg·mL-1),经实验验证,其NA活性抑制作用强于SHL(P＜0.05).Box-Behnken响应曲面设计法具有普筛高效、预测准确等优势,适合用于组分中药组方的筛选与优化配比研究,为有效推进现代组分中药的发展提供了技术支持.
- [1]M. Potier,L. Mameli,M. Bélisle,etc.Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-α-d-N-acetylneuraminate) substrate[J].1979,94(2).
- [2]Wutzler P,Bauer K,Schmidtke M,etc.Different neuraminidase inhibitor susceptibilities of human H1N1, H1N2, and H3N2 influenza A viruses isolated in Germany from 2001 to 2005/2006.[J].2009,82(1).
- [3]Yan S,Xia X,Jin H,etc.Analysis of the bioactive constituents of ChanSu in rat plasma by high performance liquid chromatography with mass spectrometric detection.[J].2010,53(3).
- [4]Borisy AA,Elliott PJ,Hurst NW,etc.Systematic discovery of multicomponent therapeutics.[J].2003,100(13).
- [5]Ferreira SLC,Bruns RE,Ferreira HS,etc.Box-Behnken design: An alternative for the optimization of analytical methods[J].2007,597(2).
- [6]Phillipson JD.Phytochemistry and pharmacognosy[J].2007,68(22/24).
- [7]Macka M,Hughes J,Ragonese R,etc.The use of the Box-Behnken experimental design in the optimisation and robustness testing of a capillary electrophoresis method for the analysis of ethambutol hydrochloride in a pharmaceutical formulation.[J].2002,27(6).
- [8]Nayak DP,Reichl U.Neuraminidase activity assays for monitoring MDCK cell culture derived influenza virus.[J].2004,122(1).
- [9]de Jesus, RM,Ferreira, SLC,Queiroz, AFD,etc.Determination of total arsenic and arsenic (III) in phosphate fertilizers and phosphate rocks by HG-AAS after multivariate optimization based on Box-Behnken design[J].2009,80(2).
- [10]Phillipson JD..Phytochemistry and medicinal plants[J].2001,56(3).
- [11]Chen Q,Zhu LC,Wang BC.Primary study on the application of Serum Pharmacology in Chinese traditional medicine[J].2005,43(3).
- [12]Zhenya Zhang,Qiang Xue,Norio Sugiura,etc.Optimization of process parameters for electrochemical nitrate removal using Box-Behnken design[J].2010,56(1).
- [13]Khajeh, M.,Sanchooli, E..Optimization of microwave-assisted extraction procedure for zinc and iron determination in celery by Box-Behnken design.[J].2010,3(2).