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Indirubin inhibits ATP-induced phagocytosis attenuation,ROS production and cell death of macrophages
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- Author:
- No author available
- Journal Title:
- Acta Pharmaceutica Sinica
- Issue:
- 1
- DOI:
- No doi available
- Key Word:
- 靛玉红;ATP;巨噬细胞;免疫反应;P2受体
Abstract： 为研究靛玉红对ATP引起的巨噬细胞免疫反应的影响,采用中性红摄入法检测细胞吞噬功能,用MTT法检测细胞活力,通过DHE探针检测ROS的产生.结果表明:细胞外ATP可抑制巨噬细胞吞噬、导致细胞死亡并促进ROS产生,靛玉红对ATP引起的以上免疫反应有抑制作用.为了解靛玉红的作用机制,进一步研究其对ATP引起的[Ca2+]i升高和P2X7受体介导的膜通透性增加的影响.结果显示:靛玉红可降低ATP引起的[Ca2+]i升高,抑制ATP诱发的EB进入.结果提示,靛玉红对P2X7的活化有阻断作用,其对ATP引起的免疫反应的抑制效果可能是通过抑制P2X7受体实现的.
- [1]O K, Nihei,A C, de Carvalho,W, Savino,etc.Pharmacologic properties of P(2Z)/P2X(7)receptor characterized in murine dendritic cells: role on the induction of apoptosis.[J].2000,96.
- [2]MacKenzie AB,Moore SF.NADPH oxidase NOX2 mediates rapid cellular oxidation following ATP stimulation of endotoxin-primed macrophages.[J].2009,183(5).
- [3]White N,Burnstock G.P2 receptors and cancer.[J].2006,27(4).
- [4]Qu Y,Zhang A,Peng J,etc.The different effects of indirubin on effector and CD4(+)CD25 (+) regulatory T cells in mice: potential implication for the treatment of autoimmune diseases.[J].2007,85(11).
- [5]Mendes AN,Coutinho Silva R,Gorecki DC,etc.Multiple P2X and P2Y receptor subtypes in mouse J774, spleen and peritoneal macrophages.[J].2005,69(4).
- [6]Iwaki K,Kunikata T,Kurimoto M,etc.Indirubin inhibits inflammatory reactions in delayed-type hypersensitivity.[J].2000,410(1).
- [7]Burnstock G,Ralevic V.Receptors for purines and pyrimidines.[J].1998,50(3).
- [8]Coutinho Silva R,Burnstock G,Ojcius DM,etc.Purinergic receptor agonists modulate phagocytosis and clearance of apoptotic cells in macrophages.[J].2011,216(1).
- [9]Son MS,Kang H,Jung HJ,etc.Indirubin-3'-oxime inhibits inflammatory activation of rat brain microglia.[J].2011,487(2).
- [10]Khakh BS,North RA.P2X receptors as cell-surface ATP sensors in health and disease[J].2006,442(7102).
- [11]Jursik,Gu BJ,Saunders BM.The P2X7-nonmuscle myosin membrane complex regulates phagocytosis of nonopsonized particles and bacteria by a pathway attenuated by extracellular ATP.[J].2010,115(8).
- [12]Lee MJ,Kim MY,Mo JS,etc.Indirubin-3'-monoxime, a derivative of a Chinese anti-leukemia medicine, inhibits Notch1 signaling.[J].2008,265(2).
- [13]Perregaux DG,Gabel CA,Solle M,etc.Altered cytokine production in mice lacking P2X(7) receptors.[J].2001,276(1).
- [14]Liu X,Zou J,Liu L,etc.Inhibition of ATP-induced macrophage death by emodin via antagonizing P2X7 receptor.[J].2010,640(1).
- [15]Kim, S. A.,Kwon, S. M.,Kim, J. A.,etc.5prime-Nitro-indirubinoxime, an indirubin derivative, suppresses metastatic ability of human head and neck cancer cells through the inhibition of Integrin b1/FAK/Akt signaling[J].2011,306(2).