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Simultaneous determination of 11 opiates in hair by liquid chromatography-tandem mass spectrometry
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- Author:
- No author available
- Journal Title:
- Acta Pharmaceutica Sinica
- Issue:
- 12
- DOI:
- No doi available
- Key Word:
- 头发;阿片类生物碱;LC-MS/MS
Abstract： 建立头发中海洛因、吗啡、单乙酰吗啡等11种阿片类生物碱的液相色谱-串联质谱测定方法,并考察海洛因滥用者头发中阿片类组分的存在情况.头发经冷冻研磨后加入硼酸缓冲液超声30 min,用氯仿-异丙醇(9∶1)提取.用Allure PFP丙基柱,以乙腈-乙酸铵(0.1％甲酸)梯度洗脱分离,采用二级质谱多反应监测模式(MRM)检测11种阿片类生物碱.头发中海洛因、吗啡、单乙酰吗啡等11种阿片类生物碱在对应质量浓度范围内线性良好(r＞ 0.996 0);检测限(LOD)均小于0.05 ng·mg-1；回收率范围为47.2％～110％;日内精密度和日间精密度均小于14％.21例海洛因滥用者头发中均检出了海洛因、单乙酰吗啡、吗啡、可待因、乙酰可待因、氢可酮等主要组分.所建方法灵敏度高、选择性好,适用于同时分析头发中海洛因等11种阿片类生物碱组分,可有效鉴别海洛因滥用与阿片类药物或食品的摄取.
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