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Sodium nitrite induces epithelial-mesenchymal transition of SMMC-7721 cells
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 5
- DOI:
- No doi available
- Key Word:
- 亚硝酸钠;人肝癌细胞;细胞因子;上皮-间质转化
Abstract： 探讨亚硝酸盐诱导人肝癌SMMC-7721细胞发生上皮-间质转化(EMT)的作用.0.25～25 mmol·L<'-1>亚硝酸钠处理细胞48 h后,酶联免疫吸附实验(ELISA)检测细胞培养上清液中的TGF-β1、IL-6和IL-8水平,相差倒置显微镜下观察细胞形态学变化,划痕实验和Transwell侵袭实验检测细胞移动和侵袭能力,同时采用Western blotting方法检测EMT相关标记蛋白和p-AKT及下游信号分子的蛋白表达水平.结果表明,亚硝酸钠处理人肝癌细胞48 h,可以引起TGF-β1的分泌显著升高,并呈现剂量依赖性.亚硝酸钠对IL-8及IL-6的分泌也具有一定的增加作用,但不具有剂量依赖性.0.25 mmol·L<'-1>亚硝酸钠作用48 h,诱导细胞形态向间质转化,增强波形蛋白和p-AKT及其下游信号蛋白的表达,抑制E型钙黏素蛋白的表达,促进细胞迁移和侵袭能力.结果提示,亚硝酸钠可能通过促进细胞分泌TGF-β1和IL-8,诱导人肝癌SMMC-7721细胞上皮-间质转化.
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