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The changes of iNOS and NO in the osteogenic differentiation process of rat bone marrow stromal cells promoted by icariside Ⅱ
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 4
- DOI:
- No doi available
- Key Word:
- 淫羊藿次苷Ⅱ;骨髓间充质干细胞;成骨性分化;诱导性一氧化氮合酶;一氧化氮
Abstract： 研究淫羊藿次苷Ⅱ(icariside Ⅱ,ICS Ⅱ)对大鼠体外培养骨髓间充质干细胞(rat bone marrow stromal cells,rBMSCs)成骨性分化过程中诱导性一氧化氮合酶(induced nitric oxide synthase,iNOS)表达及NO生成的影响.贴壁筛选法体外培养rBMSCs,待铺满80%皿底时,进行成骨性诱导培养,同时采用1×10-5mol·L-1 ICS Ⅱ进行药物干预,比较ICS Ⅱ组、L-NAME组、ICS Ⅱ+L-NAME组和不加药的对照组之间的iNOS的活性、NO生成量,对比各组之间的成骨性指标,包括碱性磷酸酶活性、碱性磷酸酶阳性克隆数(CFU-FALP)及钙化结节数量.提取总RNA,实时荧光定量RCR(real-time PCR)检测Osterix(OSX)、Runx-2及iNOS mRNA的表达情况;同时提取总蛋白,Western blotting法检测I型胶原蛋白和iNOS的分泌量.ICS Ⅱ可显著增强碱性磷酸酶(ALP)活性,增加钙化结节和CFU-FALP数量,与成骨性分化相关的因子OSX和Runx-2的基因表达量也显著升高,同时I型胶原的分泌量也明显增多,但这些效应均可被iNOS的特异性抑制剂L-NAME所抑制.ICS Ⅱ可显著促进rBMSCs的成骨性分化,但采用L-NAME进行阻断后,随着iNOS和NO表达的降低,成骨性分化的指标随之降低,提示ICS Ⅱ是通过提高iNOS活性,促进NO的生成来刺激rBMSCs的成骨性分化的.
- [1]翟远坤,李志锋,程国政,等.淫羊藿苷抗骨质疏松研究进展[J].2009,(7).
- [2]李雅琳,肖洲生.Runx2基因调控及其异构体的最新研究进展[J].2006,(10).
- [3]Dan-yan ZHU,i-jia LOU.Icariin-mediated expression of cardiac genes and modulation of nitric oxide signaling pathway during differentiation of mouse embryonic stem cells into cardiomyocytes in vitro[J].2006,(3).
- [4]邱峰,陈英杰,鹿野美弘,等.淫羊藿苷在大鼠体内的代谢[J].1999,(3).
- [5]翟远坤,葛宝丰,马慧萍,等.淫羊藿苷促进体外培养大鼠骨髓间充质干细胞的成骨性分化[J].2010,(23).
- [6]Ge BF,Bai MH,Chen KM,etc.Icariin, a flavonoid from the herb Epimedium enhances the osteogenic differentiation of rat primary bone marrow stromal cells.[J].2005,60(12).
- [7]张奕华,彭司勋.一氧化氮供体型新药研究[J].2009,(11).
- [8]Qin L,Han T,Zhang Q,etc.Antiosteoporotic chemical constituents from Er-Xian Decoction, a traditional Chinese herbal formula.[J].2008,118(2).
- [9]Li H,Zhang Y,Zhang X,etc.LC-MS/MS method for the simultaneous determination of icariin and its major metabolites in rat plasma.[J].2007,45(4).
- [10]Liu M,Xiong ZL,Liu HP,etc.Simultaneous determination of icariin, icariside II and osthole in rat plasma after oral administration of the extract of Gushudan (a Chinese compound formulation) by LC-MS/MS[J].2007,860(1).
- [11]Wu-Jiang Liu,hong-Cheng Xin,ua Xin,etc.Effects of icariin on erectile function and expression of nitric oxide synthase isoforms in castrated rats[J].2005,(4).