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Establishment and drug susceptibility test of isoniazid resistant Mycobacterium smegmatis
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 4
- DOI:
- No doi available
- Key Word:
- 耻垢分枝杆菌;inhA过表达;异烟肼耐药;评价方法
Abstract： 耐药结核病的涌现,使发展新型抗耐药结核药物变得尤为迫切.本研究选择生长快且无致病性的耻垢分枝杆菌为研究对象,探索快速评价药物抗异烟肼耐药结核分枝杆菌的能力.inhA是异烟肼的作用靶点,由于inhA的突变或者过表达可以引起结核分枝杆菌对异烟肼耐药性的产生.通过将inhA克隆入pMV261中,构建过表达inhA的耻垢分枝杆菌.结果显示,过量表达inhA的耻垢分枝杆菌对异烟肼的敏感性下降了100倍以上.建立了利用刃天青为指示剂的抗异烟肼耐药株的快速药效评价方法,可快速对药物的活性进行定性或定量评价,为进行新型抗结核药物的高通量筛选和药效评价奠定基础.
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