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Salvianolic acid B alleviate the disruption of blood-brain barrier in rats after cerebral ischemia-reperfusion by inhibiting MAPK pathway
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 12
- DOI:
- No doi available
- Key Word:
- 丹酚酸B;脑缺血再灌注;血脑屏障;MAPK通路
Abstract： 本研究旨在观察丹酚酸B对脑缺血再灌注损伤大鼠血脑屏障的影响,探讨其可能的作用机制.研究采用线栓法阻塞大鼠大脑中动脉,并于缺血后2 h进行再灌注,造成脑缺血再灌注损伤,用免疫组织化学法检测缺血侧脑血管周围免疫球蛋白IgG的渗出以及MMP-9(基质金属蛋白酶-9)和NOS2(一氧化氮合酶2)的表达,用Western blotting方法检测p-p38和p-ERK1/2蛋白含量变化.结果显示,手术后第2天缺血再灌注组损伤侧脑血管周围免疫球蛋白IgG的渗出增加,表明血脑屏障功能破坏,脑组织中MMP-9和NOS2表达增加,同时p-p38和p-ERK1/2含量也明显增加.手术后第7天,缺血再灌注组脑组织中MMP-9及NOS2依然维持在同手术后第2天接近的高水平,p-p38含量高于手术后第2天,p-ERK1/2含量略低于手术后第2天.丹酚酸B(1和10 mg·kg-1)能够明显改善缺血再灌注引起IgG的渗出增加(P<0.05),同时MMP-9和NOS2表达也明显抑制(P<0.05).丹酚酸B(10 mg·kg-1)能够显著降低手术后第2天和第7天p-p38和p-ERK1/2蛋白含量(P<0.05).丹酚酸B(1mg·kg-1)在手术后第7天能显著减少p-p38的含量(P<0.05).以上结果表明,丹酚酸B的血脑屏障保护作用可能与抑制MMP-9激活相关,其中丹酚酸B抑制MAPK通路可能起重要作用.
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