You Position: Home > Paper
Sodium nitrite preconditioning protects against ethanol-induced damage in human hepatoma SMMC-7721 cells
( views:16, downloads:0 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 10
- DOI:
- No doi available
- Key Word:
- 亚硝酸钠;乙醇;SMMC-7721 细胞;细胞保护
Abstract： 研究亚硝酸钠预处理对乙醇损伤的人肝癌SMMC-7721细胞的保护作用.0.25 mmol·L-1亚硝酸钠预处理细胞24 h或4周后,再用200 mmol·L-1乙醇处理12 h.四甲基偶氮唑蓝(MTT)法测定细胞活力,FITC-Annexin V/PI流式细胞术检测细胞凋亡,Hoechst 33258和PI活细胞联染评定细胞损伤,蛋白印迹技术(Western blotting)检测缺氧诱导因子-1α(HIF-1α)和凋亡相关蛋白表达水平.结果发现,无论亚硝酸钠短暂或慢性预处理,皆可抑制由乙醇引起的细胞凋亡和过氧化损害,同时细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性升高,丙二醛(MDA)含量下降.亚硝酸钠预处理24 h的细胞再用乙醇处理时,HIF-1α表达增高,同时促凋亡相关蛋白Bax、Caspase-9、Caspase-3表达下降,凋亡抑制蛋白Bcl-2表达增加.结果表明,低剂量亚硝酸钠预处理能够抵抗乙醇诱导的人肝癌SMMC-7721细胞凋亡,其机制可能与HIF-1α表达增高有关.
- [1]Shiva S,Sack MN,Greer JJ,etc.Nitrite augments tolerance to ischemia/reperfusion injury via the modulation of mitochondrial electron transfer.[J].2007,204(9).
- [2]Hobbs AJ,Loukogeorgakis S,Okorie M,etc.Acute blood pressure lowering, vasoprotective, and antiplatelet properties of dietary nitrate via bioconversion to nitrite.[J].2008,51(3).
- [3]Calabrese EJ.Dose-response features of neuroprotective agents: an integrative summary.[J].2008,38(4).
- [4]崔占军,赵凯冰,文曙光,等.孕期酒精暴露对小鼠视皮质神经元树突棘和突触的影响[J].2010,(7).
- [5]Zaouali MA,Boncompagni E,Freitas I,etc.Hypoxia inducible factor-1alpha accumulation in steatotic liver preservation: role of nitric oxide.[J].2010,16(28).
- [6]Arezzini, Beatrice .,Giardini, Anna,Signorini, Cinzia,etc.Ethanol-induced oxidative stress: basic knowledge[J].2010,5(2).
- [7]Hord NG,Tang Y,Bryan NS.Food sources of nitrates and nitrites: the physiologic context for potential health benefits.[J].2009,90(1).
- [8]Cho KH,Lee SM.Altered activity of cytochrome P450 in alcoholic fatty liver exposed to ischemia/reperfusion.[J].2007,30(1).
- [9]Hsu PP,Sabatini DM.Cancer cell metabolism: Warburg and beyond[J].2008,134(5).
- [10]Ahluwalia, A,Fago, A,Friedman, J,etc.Nitrate and nitrite in biology, nutrition and therapeutics[J].2009,5(12).
- [11]Ajit Bharti,Selena M Bauer,Ronald E Maloney,etc.Nitrite is a signaling molecule and regulator of gene expression in mammalian tissues[J].2005,1(5).
- [12]Kim JW,Semenza GL,Dang CV,etc.Hypoxia-inducible factor I and dysregulated c-myc cooperatively induce vascular endothelial growth factor and metabolic switches hexokinase 2 and pyruvate dehydrogenase kinase 1[J].2007,27(21).
- [13]Rahman MM,Kim SJ,Kim GB,etc.Nitrite-induced methemoglobinaemia affects blood ionized and total magnesium level by hydrolysis of plasma adenosine triphosphate in rat.[J].2009,105(5).
- [14]Raat NJ,Liu VB,Noguchi AC.Dietary nitrate and nitrite modulate blood and organ nitrite and the cellular ischemic stress response.[J].2009,47(5).
- [15]Vowinkel, Thorsten,Saijo, Fumito,Ivanovic, Marina,etc.On the dynamics of nitrite, nitrate and other biomarkers of nitric oxide production in inflammatory bowel disease[J].2010,22(2).
- [16]张奕华,彭司勋.一氧化氮供体型新药研究[J].2009,(11).