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Advances in the study of affinity selection-ultrafiltration/HPLC-MS

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Author:: No author available

Journal Title:: ACTA PHARMACEUTICA SINICA

Issue:: 10

DOI:: 10.3321/j.issn:0513-4870.2009.10.004

Key Word:: 亲和超滤HPLC-MS法;小分子活性物质;筛选和发现药物;药物研究

Abstract：亲和超滤HPLC-MS法是将超滤技术与HPLC-MS联用,主要用于从组合化学库和天然产物粗提物中筛选与可溶性靶点相结合的小分子活性物质,在药物代谢和描述受体-配体结合特征两方面也有所应用.用该方法筛选和发现药物被认为是传统药物发现方法的一种补充.本文综述了亲和超滤HPLC-MS法的原理及其在药物研究中的应用.

Reference

[1]Geun Shin Y,Bolton JL,van Breemen RB.Screening drugs for metabolic stability using pulsed ultrafiltration mass spectrometry.[J].2002,5(1).
[2]M M, Siegel,K, Tabei,G A, Bebernitz,etc.Rapid methods for screening low molecular mass compounds non-covalently bound to proteins using size exclusion and mass spectrometry applied to inhibitors of human cytomegalovirus protease.[J].1998,33.
[3]van-Breemen RB,Johnson BM,Bolton JL.Screening botanical extracts for quinoid metabolites.[J].2001,14(11).
[4]Claude G, Lerner,Bruce A, Beutel.Antibacterial drug discovery in the post-genomics era.[J].2002,2(2).
[5]C, Gu,D, Nikolic,J, Lai,etc.Assays of ligand-human serum albumin binding using pulsed ultrafiltration and liquid chromatography-mass spectrometry.[J].1999,2.
[6]Park C,Coen M,Comess KM,etc.Kinase drug discovery by affinity selection/mass spectrometry (ASMS): Application to DNA damage checkpoint kinase Chk1[J].2006,11(7).
[7]R B, van Breemen,C R, Huang,D, Nikolic,etc.Pulsed ultrafiltration mass spectrometry: a new method for screening combinatorial libraries.[J].1997,69.
[8]Comess KM,Schurdak ME,Voorbach MJ,etc.An ultraefficient affinity-based high-throughout screening process: Application to bacterial cell wall biosynthesis enzyme MurF[J].2006,11(7).
[9]C J, Chen,S, Chen,C P, Woodbury,etc.Pulsed ultrafiltration characterization of binding.[J].1998,261.
[10]Beverly MB,West P,Julian RK.Evaluation of a micro volume pulsed ultrafiltration cell for screening ligands in non-covalent complexes.[J].2002,5(1).
[11]Nikolic D.,van Breemen RB.,Johnson BM..Applications of pulsed ultrafiltration-mass spectrometry [Review][J].2002,21(2).
[12]Schurdak ME,Comess KM.Affinity-based screening techniques for enhancing lead discovery.[J].2004,7(4).
[13]van-Breemen RB,Bolton JL,Nikolic D.Metabolic screening using on-line ultrafiltration mass spectrometry.[J].1998,26(2).
[14]Yao P,Liang WZ,Bolton JL,etc.Ultrafiltration tandem mass spectrometry of estrogens for characterization of structure and affinity for human estrogen receptors[J].2005,16(2).
[15]Ray Wieboldt,Jerry Zweigenbaum,Jack Henion.Immunoaffinity Ultrafiltration with lon Spray HPLC/MS for Screening Small-Molecule Libraries[J].1997,69(9).
[16]Annis DA,Nickbarg E,Yang X,etc.Affinity selection-mass spectrometry screening techniques for small molecule drug discovery[J].2007,11(5).
[17]Richard B. Van Breemen,John M. Pezzuto,Sohrab Habibi-Goudarzi,etc.Evaluation of Cyclooxygenase-2 Inhibitors Using pulsed Ultrafiltration Mass Spectrometry[J].2000,72(16).