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Establishment of a diabetic-hypertensive rat model
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0513-4870.2009.06.004
- Key Word:
- 高血压;糖尿病;高盐
Abstract： 本实验旨在建立一种糖尿病-高血压大鼠模型.采用Wistar大鼠腹腔注射STZ(链脲佐菌素)诱导糖尿病之后,给予1%NaCl饮水,以无创尾动脉血压测定法进行血压监测.至血压稳定高于正常大鼠50 mmHg后,颈动脉插管法测定血压,测定血浆ET-1、Ang II含量;并采用半定量PCR方法对肾脏、肝脏、主动脉血管紧张素原基因表达及前内皮素原基因表达情况进行检测.结果显示,STZ诱导的糖尿病大鼠给予1%NaCl饮水3周,血压 开始升高,第6周升至约150mmHg,并维持5周左右,至第12周血压继续上升到160mmHg以上.此时,大鼠伴有血浆ET-1含量和肾脏Ang II含量上升,丰动脉ET-1和肾脏Ang II基因表达上调.因此,STZ诱导糖尿病大鼠饮用1%NaCl可制备一种糖尿病相关的高血压动物模型.
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