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Preparation and application of antibody against staphylococcal enterotoxin C2
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 8
- DOI:
- No doi available
- Key Word:
- 金葡菌肠毒素C2;单克隆抗体;生物素-亲和素酶联免疫吸附实验
Abstract： 生产厂家以金葡菌肠毒素C2(SEC2)的标示量作为金葡素注射液的质量控制标准,但其真正的含量由于滤液成分复杂而很难检测,本实验试图建立一种方便的鉴定金葡素注射液中的SEC2的方法.首先将重组的SEC2分别免疫BALB/c小鼠和新西兰兔,制备并纯化了单克隆及多克隆抗体.采用自制的抗体建立了检测SEC2的生物素一链霉亲和素酶联免疫吸附实验,检测目标蛋白的质量浓度范围为2～20 ng·mL-1,在检测范围内检测值的平均变异系数为5.08%,采用本法检测质量浓度分别为100 ng·mL-1的重组肠毒素(SEA/SEO/SEM/SEN/SEG/SEI),均呈现阴性反应,说明此检测方法的专一性较好.采用此方法对金葡素注射液中的SEC2含量进行了检测,发现SEC2标识量与实际含量存在一定的差距.
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