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Exendin-4 protected murine MIN6 pancreatic β-cells from oxidative stress-induced apoptosis via down-regulation of NF-κB-iNOS-NO pathway
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 7
- DOI:
- 10.3321/j.issn:0513-4870.2008.07.004
- Key Word:
- Exendin-4;胰岛β细胞;细胞凋亡;核因子κB;诱导型一氧化氮合酶;一氧化氮;叔丁基过氧化氢
Abstract： 探讨胰高血糖素样肽-1受体激动剂Exendin-4(Ex-4)在氧化损伤诱导胰岛β细胞凋亡中的保护作用.培养的MIN6胰岛β细胞,通过AO-EB染色观察细胞凋亡形态,Annexin-V-PI染色流式技术测定凋亡率,Griess法检测细胞内一氧化氮水平,Western blotting检测胞浆iNOS蛋白、胞浆及胞核核因子-κBp65(NF-κBp65)蛋白表达水平.Ex-4可抑制叔丁基过氧化氢(t-BHP)诱导的β细胞凋亡,Ex-4(100nmol·L-1)预处理较单独t-BHP处理,其凋亡率减少约67%(P<0.001).Ex-4同时减少NO水平的增高,并抑制t-BHP诱导的β细胞NF-κBp65活化及iNOS蛋白表达水平.Ex-4可能通过抑制细胞内NF-κB活化、胞浆iNOS表达来抑制NO水平,最终减轻氧化损伤诱导的β细胞凋亡.
- [1]李强,许霖水.外源一氧化氮对大鼠胰岛细胞损伤机制研究[J].2002,(5).
- [2]H, Kaneto,J, Fujii,H G, Seo,etc.Apoptotic cell death triggered by nitric oxide in pancreatic beta-cells.[J].1995,44.
- [3]Baldwin AS.Series introduction: the transcription factor NF-kappaB and human disease.[J].2001,107(1).
- [4]Hussain MA,Bonner Weir-S,Stoffers DA,etc.Insulinotropic glucagon-like peptide 1 agonists stimulate expression of homeodomain protein IDX-1 and increase islet size in mouse pancreas.[J].2000,49(5).
- [5]E, Schreiber,P, Matthias,M M, Müller,etc.Rapid detection of octamer binding proteins with 'mini-extracts', prepared from a small number of cells.[J].1989,17.
- [6]O'Brien T,McCabe C,Samali A.beta cell cytoprotective strategies: Establishing the relative roles for iNOS and ROS[J].2006,342(4).