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Effect of Nordy on the function of endothelial progenitor cells from human umbilical cord blood induced by vascular endothelial growth factor
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0513-4870.2008.02.005
- Key Word:
- 内皮祖细胞;血管内皮生长因子;诺帝
Abstract： 探讨手性化合物诺帝(Nordy)对血管内皮生长因子(VEGF)诱导的人脐血源性内皮祖细胞(EPCs)功能的影响及其意义.应用密度梯度离心法分离新鲜人脐血的单个核细胞,接种于EGM-2培养液中培养7～10 d获得内皮祖细胞(EPCs).分别采用MTT法、Millicell-PCF培养小室系统和Matrigel内小管形成试验检测诺帝对VEGF刺激下EPCs增殖活性、迁移能力和体外形成小管样结构能力的影响.结果表明,100 μmol·L-1诺帝作用24 h明显抑制EPCs增殖活性(P<0.05),诺帝(25～50 μmol·L-1)作用48～72 h也明显抑制EPCs增殖活性(P<0.05).诺帝(25～100 μmol·L-1)显著抑制VEGF诱导的EPCs迁移活性和体外形成小管样结构的能力(P<0.05).诺帝能抑制体外VEGF诱导的人脐血源性EPCs增殖、迁移和体外小管形成能力,提示其具有抗EPCs效应.
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