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Process of HIV-1 reverse transcription and its detection by using PCR

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Author:: No author available

Journal Title:: ACTA PHARMACEUTICA SINICA

Issue:: 2

DOI:: 10.3321/j.issn:0513-4870.2008.02.002

Key Word:: HIV-1;逆转录;聚合酶链反应

Abstract：人类免疫缺陷病毒(human immunodeficiency virus,HIV)作为逆转录病毒,在进入宿主细胞以后其正链RNA基因组就在逆转录酶的作用下开始逆转录合成双链的DNA.HIV-1逆转录的过程大致包括以下几个步骤:负链强力终止DNA的起始合成,负链强力终止DNA的链转移,正链DNA的合成起始,正链强力终止DNA的链转移和最终完整双链DNA的合成.逆转录是HIV-1复制的重要环节,现已成为抗HIV-1药物研究开发的重要靶标.聚合酶链反应(polymerase chain reaction,PCR)可用来检测HIV-1的逆转录过程,设计引物扩增HIV-1基因组R/U5序列、U3序列、U5/PBS序列和LTR与Gag之间的序列,根据PCR结果可相应判断出HIV-1的逆转录是否起始、是否进行了负链的链转移、正链的起始和双链DNA的完整合成.本文将主要介绍逆转录过程以及应用PCR的检测方法.

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