You Position: Home > Paper
Protective effect of (-)clausenamide against neurotoxicity induced by okadaic acid and β-amyloid peptide25-35
( views:204, downloads:12 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 9
- DOI:
- 10.3321/j.issn:0513-4870.2007.09.005
- Key Word:
- 左旋黄皮酰胺;阿尔茨海默病;冈田酸;β淀粉样肽25-35;神经保护作用
Abstract： 探讨左旋黄皮酰胺对冈田酸(okadaic acid,OA)诱导的人神经瘤细胞(SH-SY5Y)和去卵巢(ovariectomy,OVX)及单侧侧脑室注射Aβ25-35所致神经元损伤的保护作用.通过MTT试验、LDH释放测定试验、Hoechst 33258荧光染色试验以及SH-SY5Y细胞检测,考察左旋黄皮酰胺拮抗冈田酸诱导的细胞毒作用.通过避暗试验、电镜检测、Nissl体染色及HE染色,考察左旋黄皮酰胺对去卵巢及侧脑室注射Aβ25-35大鼠神经元的保护作用.左旋黄皮酰胺可明显拮抗冈田酸诱导的细胞毒作用,提高去卵巢及侧脑室注射Aβ25-35大鼠的学习记忆能力,保护海马及皮层神经元.左旋黄皮酰胺可拮抗冈田酸及Aβ25-35诱导的神经毒性,具有神经保护作用.
- [1]曾育琦,陈晓春,朱元贵,等.人参皂苷Rb1抑制β淀粉样蛋白25-35诱导的皮层神经元tau蛋白过度磷酸化[J].2005,(3).
- [2]Gema Alvarez,Juan Ramón Muñoz-Montaño,Jorgina Satrústegui,etc.Lithium protects cultured neurons against β-amyloid-induced neurodegeneration[J].1999,453(3).
- [3]Gong CX,Wegiel J,Zuck L,etc.Phosphorylation of microtubule-associated protein tau is regulated by protein phosphatase 2A in mammalian brain. Implications for neurofibrillary degeneration in Alzheimer's disease.[J].2000,275(8).
- [4]D J, Selkoe.The molecular pathology of Alzheimer's disease.[J].1991,6(4).
- [5]Chuang DM.Neuroprotective and neurotrophic actions of the mood stabilizer lithium: can it be used to treat neurodegenerative diseases?[J].2004,16(1).
- [6]S. Lovestone,C.L. Hartley,J. Pearce,etc.Phosphorylation of tau by glycogen synthase kinase-3β in intact mammalian cells: The effects on the organization and stability of microtubules[J].1996,73(4).
- [7]Zhang JT,Tang K.-)Clausenamide improves long-term potentiation impairment and attenuates apoptosis after transient middle cerebral artery occlusion in rats.[J].2003,25(7).
- [8]J W, Simpkins,P S, Green,K E, Gridley,etc.Role of estrogen replacement therapy in memory enhancement and the prevention of neuronal loss associated with Alzheimer's disease.[J].1997,103.
- [9]Gema Alvarez,Juan Ramón Muñoz-Montaño,Jorgina Satrústegui,etc.Regulation of tau phosphorylation and protection against β-amyloid-induced neurodegeneration by lithium. Possible implications for Alzheimer's disease[J].2002,4(3).
- [10]M, Hong,D C, Chen,P S, Klein,etc.Lithium reduces tau phosphorylation by inhibition of glycogen synthase kinase-3.[J].1997,272.
- [11]T, Tanaka,J, Zhong,K, Iqbal,etc.The regulation of phosphorylation of tau in SY5Y neuroblastoma cells: the role of protein phosphatases.[J].1998,426.
- [12]Goodenough S,Behl C,Schafer M.Estrogen-induced cell signalling in a cellular model of Alzheimer's disease.[J].2003,84(2).