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In vitro anti-angiogenic action of λ-carrageenan oligosaccharides
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 6
- DOI:
- 10.3321/j.issn:0513-4870.2007.06.005
- Key Word:
- λ-卡拉胶;寡糖;血管生成;鸡胚尿囊膜;脐静脉内皮细胞
Abstract： 为探讨λ-卡拉胶寡糖体外对血管生成的抑制作用,采用鸡胚尿囊膜模型(CAM)观察λ-卡拉胶寡糖对CAM血管发育的抑制,发现该寡糖可明显抑制CAM微血管生成,在200 μg·egg-1时,抑制率达54.90%.随后采用MTT法测定λ-卡拉胶寡糖对人脐静脉内皮细胞(HUVEC)增殖的影响,发现该寡糖的细胞毒作用在不同的细胞间具有选择性,对HUVEC的抑制最强,高浓度时(1 mg·mL-1)能明显抑制其增殖,但低浓度(＜250 μg·mL-1)对细胞几乎无毒.对寡糖的细胞迁移和侵袭抑制作用进行检测,并测定其对HUVEC表达基质金属蛋白酶-2(MMP-2)的影响.结果表明,λ-卡拉胶寡糖能有效减缓HUVEC的迁移、侵袭能力,并具有抑制HUVEC中MMP-2表达的作用.该研究说明λ-卡拉胶寡糖通过抑制内皮细胞的增殖、细胞侵袭和迁移达到血管生成抑制的作用.
- [1]马恩龙,李艳春,王敏伟.血管形成和血管形成抑制剂的应用[J].2005,(2).
- [2]杨军,江敏,甄永苏.新生霉素抑制血管生成及其与长春新碱的协同作用[J].2003,(10).
- [3]陈欢欢,周慧君.青蒿琥酯的抗血管生成作用[J].2004,(1).
- [4]Leali D,Presta M,Liekens S,etc.Heparin derivatives as angiogenesis inhibitors.[J].2003,9(7).
- [5]Lawnicka H,Jerczynska H,Stepien H,etc.Effect of thalidomide affecting VEGF secretion, cell migration, adhesion and capillary tube formation of human endothelial EA.hy 926 cells[J].2006,78(22).
- [6]M. Luescher-Mattli.Algae, A Possible Source for New Drugs in the Treatment of HIV and Other Viral Diseases[J].2003,2(3).
- [7]Soeda S,Shimeno H,Koyanagi S,etc.Oversulfation of fucoidan enhances its anti-angiogenic and antitumor activities.[J].2003,65(2).
- [8]Dietrich H. Paper,Horst Vogl,G. Franz,etc.Defined carrageenan derivatives as angiogenesis inhibitors[J].1995,99(1).
- [9]Wierzba MK.,Teruya T.,Osada H.,etc.Novel heparan sulfate mimetic compounds as antitumor agents[J].2004,11(3).
- [10]C R, Parish,D R, Coombe,K B, Jakobsen,etc.Evidence that sulphated polysaccharides inhibit tumour metastasis by blocking tumour-cell-derived heparanases.[J].1987,40.
- [11]Christopher M.Hattan,Cristina Fernandez,Robert J.Kerns.Semi-synthetic heparin derivatives:chemical modifications of heparin beyond chain length, sulfate substitution pattern and N-sulfo/N-acetyl groups[J].2006,341(10).
- [12]Kim J,Kwon BS,Son KN,etc.Human CC chemokine CCL23 enhances expression of matrix metalloproteinase-2 and invasion of vascular endothelial cells.[J].2006,340(2).
- [13]C R, Parish,C, Freeman,K J, Brown,etc.Identification of sulfated oligosaccharide-based inhibitors of tumor growth and metastasis using novel in vitro assays for angiogenesis and heparanase activity.[J].1999,59.