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Effects of matrine, oxymatrine and resveratrol on HERG channel expression
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 2
- DOI:
- 10.3321/j.issn:0513-4870.2007.02.005
- Key Word:
- 苦参碱;氧化苦参碱;白藜芦醇;HERG
Abstract： 由于HERG钾通道在心律失常的发生与治疗中具有双重作用,因此成为近年来研究的热点.本文主要通过免疫荧光化学染色法、激光共聚焦显微镜及Western blotting法分别定性、定量地检测不同浓度的苦参碱、氧化苦参碱和白藜芦醇对稳定转染在HEK-293细胞中HERG钾通道表达的影响.研究发现,苦参碱(1 μmol·L-1)和氧化苦参碱(1 μmol·L-1)均能够促进定位于HERG-HEK细胞膜上的HERG钾通道的表达(n=5,P＜0.05),苦参碱(100 μmol·L-1)能够抑制定位于HERG-HEK细胞膜上的HERG钾通道表达(n=5,P＜0.05),氧化苦参碱(100 μmol·L-1)对HERG钾通道表达没有影响,白藜芦醇不影响HERG-HEK细胞中HERG钾通道表达.结果表明,低浓度的苦参碱促进HERG钾通道表达,高浓度的苦参碱抑制HERG钾通道表达,低浓度的氧化苦参碱促进HERG钾通道表达,白藜芦醇不影响HERG钾通道表达.这为苦参碱、氧化苦参碱和白藜芦醇抗心律失常作用机制及安全性提供了理论依据.
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