You Position: Home > Paper
Resveratrol restored the structural and functional association between M3 receptor and connexin 43 gap junction proteins in ischemia-reperfusion injury of isolated rat heart
( views:166, downloads:16 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0513-4870.2007.01.004
- Key Word:
- 白藜芦醇;缺血再灌注损伤;M3受体;间隙连接蛋白43
Abstract： 为了探讨白藜芦醇是否能通过影响M3受体和间隙连接蛋白43(Cx43)间结构及功能性整合发挥其抗心肌缺血再灌注损伤作用,应用免疫共沉淀、免疫印迹及免疫荧光技术研究白藜芦醇对M3受体与Cx43间结构及功能性整合的影响.结合大鼠离体II导联心电图及心肌超氧化物歧化酶(SOD)、丙二醛(MDA)的检测观察白藜芦醇是否能恢复心肌缺血再灌注损伤.白藜芦醇能修复心肌缺血再灌注损伤所致的M3受体与Cx43间结构及功能性整合的破坏及纠正Cx43表达异常.同时QRS波时限﹑SOD及MDA的改变也得到相应恢复.白藜芦醇能修复M3受体与Cx43间结构及功能性整合而发挥抗缺血再灌注损伤作用.
- [1]赵兴胜,周葆初,于维汉.牛磺酸对大鼠心脏钙代谢的影响[J].2002,(5).
- [2]刘艳,孙宏丽,吴红,等.M3受体对体外H2O2诱导大鼠心肌细胞凋亡的保护作用[J].2004,(11).
- [3]肖静,岳朋,潘振伟,等.优化心肌膜蛋白提取方法及心肌缺血时间隙连接蛋白43的表达变化[J].2005,(6).
- [4]刘艳,许超千,焦军东,等.抗心律失常药物作用的新靶点--M3-R/IKM3[J].2005,(1).
- [5]杨宝峰,单宏丽,龚冬梅,等.抗心律失常药物作用最佳靶点研究[J].2002,(1).
- [6]张勇,岳朋,肖静,等.大鼠心室肌M3受体与间隙连接蛋白43作为抗心律失常药物靶点的综合研究[J].2006,(5).
- [7]Lin HX,Yang BF,Han H,etc.Choline produces cytoprotective effects against ischemic myocardial injuries: Evidence for the role of cardiac M-3 subtype muscarinic acetylcholine receptors[J].2005,16(4).
- [8]Yang B,Liu Y,Li B,etc.Resveratrol, a natural ingredient of grape skin: antiarrhythmic efficacy and ionic mechanisms.[J].2006,340(4).
- [9]Henry LA,Witt DM.Resveratrol: phytoestrogen effects on reproductive physiology and behavior in female rats.[J].2002,41(2).
- [10]Bradamante S,Barenghi L,Villa A.Cardiovascular protective effects of resveratrol.[J].2004,22(3).
- [11]Kanagaratnam P,Rothery S,Patel P,etc.Relative expression of immunolocalized connexins 40 and 43 correlates with human atrial conduction properties.[J].2002,39(1).
- [12]Guerrero P A,Schuessler R B,Davis L M,etc.Slow ventricular conduction in mice heterozygous for a connexin43 null mutation.[J].1997.
- [13]Schram G,Wang Z,Shi H,etc.Expression of multiple subtypes of muscarinic receptors and cellular distribution in the human heart.[J].2001,59(5).
- [14]S A, Thomas,R B, Schuessler,C I, Berul,etc.Disparate effects of deficient expression of connexin43 on atrial and ventricular conduction: evidence for chamber-specific molecular determinants of conduction.[J].1998,97.
- [15]J E, Saffitz,K G, Green,W J, Kraft,etc.Effects of diminished expression of connexin43 on gap junction number and size in ventricular myocardium.[J].2000,278(5).
- [16]Kizaki M,Sagawa M,Shimizu T,etc.Resveratrol induces apoptosis of human malignant B cells by activation of caspase-3 and p38 MAP kinase pathways.[J].2006,71(6).
- [17]Wang WH,Xiao J,Ma WC,etc.Ischemia impairs the association between connexin 43 and M3 subtype of acetylcholine muscarinic receptor (M3-mAChR) in ventricular myocytes[J].2006,17(3).
- [18]Buzas CJ,Seiz JL,Cook AC,etc.Inhibition of protein kinase C by resveratrol.[J].2003,1637(1).
- [19]Hao Han, Dong,He Li, Ren.New progression in the study of protective properties of resveratrol in anticardiovascular disease.[J].2004,105(5-6).