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γ-Schisandrin inhibits production of amyloid β-protein 42 in M146L cells
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 12
- DOI:
- 10.3321/j.issn:0513-4870.2006.12.003
- Key Word:
- 五味子乙素;阿尔茨海默病;β淀粉样蛋白;分泌酶抑制剂
Abstract： 目的 研究五味子乙素抑制M146L细胞Aβ42生成的机制.方法 体外培养高效表达Aβ42的M146L细胞株,分别加入不同浓度的五味子乙素(1.67,5.00和15.00 μg·mL-1)、β分泌酶抑制剂(S4562,100.00 μg·mL-1)和γ分泌酶抑制剂(S2188,13.68 μg·mL-1).用CCK-8(cell counting kit-8)比色法检测不同处理对M146L细胞活性的影响;用ELISA法测定M146L细胞所分泌的Aβ42的变化;用Western blotting检测APP的β分泌酶剪切产物C99蛋白的含量变化,结合用β和γ分泌酶活性试剂盒,检测五味子乙素对这两种酶活性的影响.结果 不同处理因素对M146L细胞的存活率均无影响,不具有细胞毒作用.中、高剂量的五味子乙素均不同程度地抑制M146L细胞分泌Aβ42及γ分泌酶的活性,但都不改变M146L细胞C99蛋白的含量及β分泌酶的活性.结论 五味子乙素可抑制γ分泌酶活性,其降低Aβ42生成是通过抑制γ分泌酶的活性来实现的.
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