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The roles of c-Jun and CBP in the inhibitory effect of quercetin on prostate cancer cells
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 9
- DOI:
- 10.3321/j.issn:0513-4870.2006.09.004
- Key Word:
- c-Jun;CBP;槲皮素;雄激素受体;前列腺癌;细胞株
Abstract： 目的 探讨槲皮素抑制前列腺癌的作用机制.方法 应用蛋白印迹技术检查槲皮素(quercetin)对雄激素受体(androgen receptor,AR)的辅调节因子c-Jun和cAMP应答元件结合蛋白的结合蛋白[cAMP response element binding protein(CREB)-binding protein,CBP]蛋白表达的影响;利用细胞转染技术检测c-Jun和CBP对AR功能的影响;免疫沉淀技术检验c-Jun与AR的蛋白-蛋白相互作用.结果 槲皮素能够明显诱导c-Jun的高表达,高表达的c-Jun能够抑制AR的功能.槲皮素对CBP的蛋白表达水平无明显影响,而增加CBP的表达并不能逆转槲皮素对AR功能的抑制作用.免疫沉淀结果表明,c-Jun与AR存在蛋白-蛋白相互作用.结论 槲皮素抑制前列腺癌的机制可能是通过c-Jun与AR的蛋白相互作用,而不是通过c-Jun竞争结合AR的辅激活因子CBP来实现的.
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