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Effect and mechanism of recombinant hirudin on fibrinolysis
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 9
- DOI:
- 10.3321/j.issn:0513-4870.2006.09.003
- Key Word:
- 重组水蛭素;纤维蛋白;凝血酶激活的纤溶抑制物;XⅢ因子
Abstract： 目的 研究重组水蛭素(recombinant hirudin,rH)在纤溶过程中的作用及其可能的机制.方法 采用同位素示踪技术,分析rH对动物血栓模型中凝血酶-纤维蛋白复合物的影响.采用tPA为纤溶激酶的体外溶栓模型,分析rH对纤溶的影响.结果 标记了99mTc的犬股动、静脉血栓于30 min开始显影,随后各时相血栓影像趋清晰;体外纤溶结果显示,rH添加组较对照组没有纤溶再凝现象.TM的添加显著延长纤溶时间,CPI可缩短由TAFI活化造成的纤溶时间的延长,rH的添加可以使TAFI的活化被抑制.较低浓度rH(≤0.2 u·mL-1)的添加使血浆中FXⅢ的活化被抑制,并且使纤溶产物D-Dimer的水平升高.结论 rH可有效抑制结合在Fn上的Th;rH可以通过抑制TAFI和FXⅢ的活化达到促进纤溶的作用.
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