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Comparison of the inhibitory activities of salvianolic acid B and Ginkgo biloba extract Egb 761 on neurotoxicity of β-amyloid peptide
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 8
- DOI:
- 10.3321/j.issn:0513-4870.2006.08.004
- Key Word:
- 丹酚酸B;阿尔茨海默病;银杏叶提取物EGb761;β-淀粉样蛋白;PC12细胞
Abstract： 目的 比较丹酚酸B(Sal B)和银杏叶提取物EGb 761对β-淀粉样蛋白(β-AP)纤维形成及细胞毒作用的影响.方法 运用硫黄素T(ThT)荧光法和电子显微镜技术分析Sal B和EGb 761对β-AP1-40聚集和纤维形成的影响;另将β-AP25-35预先老化7 d,用MTT法和流式细胞仪检测两种提取物对此老化蛋白造成的PC12细胞毒性的保护作用,用荧光法观察β-AP25-35作用后细胞内活性氧含量的变化以及两种提取物的作用.结果 Sal B和EGb 761都可有效地抑制β-AP1-40纤维的形成,明显抑制老化的β-AP25-35对PC12细胞的毒性作用,降低β-AP25-35造成的细胞内活性氧含量的增加.Sal B的有效剂量远远低于EGb 761.结论 Sal B对β-AP神经毒性的抑制作用强于EGb 761.
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