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Influence of particle size and MePEG molecular weight on in vitro macrophage uptake and in vivo long circulating of stealth nanoparticles in rats
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0513-4870.2006.04.003
- Key Word:
- 肿瘤坏死因子;纳米粒;隐形;长循环;单核吞噬细胞系统
Abstract： 目的考察粒径和单甲氧基聚乙二醇(methoxypolyethyleneglycol,MePEG)相对分子质量对重组人肿瘤坏死因子(recombinant human tumor necrosis factor-α,rHuTNF-a)隐形纳米粒体外巨噬细胞吞噬和大鼠体内长循环的影响.方法复乳法制备3种不同粒径(约为80,170和240 nm)和表面用3种不同相对分子质量MePEG(Mr为2 000,5 000和10 000)修饰的重组人肿瘤坏死因子聚乙二醇化聚十六烷基氰基丙烯酸酯[poly(methoxypolyethyleneglycol cyanoacrylate-co-n-hexadecyl cyanoacrylate),PEG-PHDCA]隐形纳米粒.进行不同纳米粒体外巨噬细胞吞噬和大鼠体内药代动力学试验,并加以比较.结果粒径相同时,随着MePEG相对分子质量的增加,巨噬细胞吞噬量减小,血浆半衰期延长;相同MePEG(Mr=5 000)修饰时,随着粒径的减小,巨噬细胞吞噬量减小,大鼠血浆半衰期延长.粒径和MePEG相对分子质量与隐形纳米粒体外巨噬细胞摄取和大鼠体内长循环性质间有良好的线性相关性.其中,PEG5 000-PHDCA纳米粒(80.0 nm)体外减小巨噬细胞吞噬和大鼠体内长循环的能力最强.结论实验范围内,粒径和MePEG相对分子质量对重组人肿瘤坏死因子隐形纳米粒体外巨噬细胞吞噬和大鼠体内长循环有显著影响.
- [1]施斌,方超,游美羡,等.隐形PEG-PHDCA纳米囊泡：PEG的相对分子质量对体外补体消耗和巨噬细胞吞噬的影响[J].2005,(11).
- [2]方超,裴元英.运载抗肿瘤药物的隐形纳米粒的研究进展[J].2004,(2).
- [3]Lim LY,Khor E,Huang M.Uptake and cytotoxicity of chitosan molecules and nanoparticles: effects of molecular weight and degree of deacetylation.[J].2004,21(2).
- [4]Hunter AC,Murray JC,Moghimi SM righton.ac.uk.Long-circulating and target-specific nanoparticles: theory to practice.[J].2001,53(2).
- [5]Qiang Zhang,Gongtie Liao,Dapen Wei,etc.Increase in gentamicin uptake by cultured mouse peritoneal macrophages and rat hepatocytes by its binding to polybutylcyanoacrylate nanoparticles[J].1998,164(1-2).
- [6]Maeda H.,Sawa T.,Matsumura Y.,etc.Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review[J].2000,65(1/2).
- [7]Y P, Li,Y Y, Pei,Z H, Zhou,etc.Stealth polycyanoacrylate nanoparticles as tumor necrosis factor-alpha carriers: pharmacokinetics and anti-tumor effects.[J].2001,24.
- [8]Devissaguet JP,Vert M,Mysiakine E,etc.Biodistribution of long-circulating PEG-grafted nanocapsules in mice: effects of PEG chain length and density.[J].2001,18(10).
- [9]Brigger I,Couvreur P,Dubernet C.Nanoparticles in cancer therapy and diagnosis.[J].2002,54(5).
- [10]Appel M,Peracchia MT,Besnard M,etc.Stealth PEGylated polycyanoacrylate nanoparticles for intravenous administration and splenic targeting.[J].1999,60(1).
- [11]Nakade A.,Sonobe T.,Sadzuka Y.,etc.Study on the characterization of mixed polyethyleneglycol modified liposomes containing doxorubicin[J].2003,91(3).