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Induction of apoptosis of tumor multidrug resistance cell by uvarigrin and its mechanism
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0513-4870.2006.03.013
- Key Word:
- 大花紫玉盘素;线粒体跨膜电位;多药抗药性;细胞凋亡
Abstract： 目的比较研究番荔枝内酯大花紫玉盘素(uvarigrin)诱导多药抗药性KBv200细胞及其亲本KB细胞凋亡及其机制.方法以MTT法进行细胞毒测定;用Annexin V FITC染色及流式细胞仪检测细胞凋亡.活性氧(ROS)测定以DCFH-DA标记,细胞线粒体跨膜电位(△Ψm)测定用DiOC6标记,均以流式细胞仪检测.Caspase-9激活的测定用Western blotting法.结果大花紫玉盘素对KBv200细胞及其亲本KB细胞的生长均有明显的抑制作用;大花紫玉盘素不仅能介导KB细胞凋亡,而且也能介导KBv200细胞凋亡;大花紫玉盘素作用于KBv200细胞及其亲本KB细胞12,24和48 h,均引起ROS升高以及△Ψm降低,而且呈时间依赖性.Western blotting方法分析显示Caspase-9被激活.结论大花紫玉盘素可能通过线粒体通路诱导细胞凋亡.
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