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Rapid analysis of antiepileptic drugs in human plasma by micellar electrokinetic capillary chromatography
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0513-4870.2006.03.004
- Key Word:
- 胶束电动毛细管色谱法;抗癫痫药物;苯妥英;苯巴比妥;卡马西平;扑米酮;氯硝西泮;丙戊酸
Abstract： 目的建立胶束电动毛细管色谱(MECC)法分析人血浆中的多种抗癫痫药物的方法.方法运行缓冲液为50 mmol·L-1SDS-8 mmol·L-1Na2HPO4-3 mmol·L-1Na2B4O7(pH 8.0)-乙腈(18%).毛细管总长50 cm,有效长度45.5 cm,内径50 μm.操作电压25 kV,运行温度30℃.检测波长210 nm.考察了各种因素对MECC分离的影响.结果线性范围:扑米酮1.0～40.0μg·mL-1,苯巴比妥1.0～60.0μg·mL,苯妥英1.0～60.0μg·mL-1,卡马西平1.0～40.0μg·mL,氯硝西泮0.2～8.0μg·mL-1,线性良好,相关系数均大于0.999 1;精密度良好,日内、日间RSD均小于15.0%;提取回收率80.0%～100.0%,RSD均小于10.0%.结论胶束电动毛细管色谱法可同时检测人血浆内多种抗癫痫药物的含量,方法成本低、操作简便、快速准确、干扰因素少.
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