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Protective effect of non-mitogenic haFGF on retinal injury induced by N-methyl-N-nitrosourea in Sprague-Dawley rats
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 4
- DOI:
- 10.3321/j.issn:0513-4870.2005.04.004
- Key Word:
- 视网膜色素变性;非促分裂人酸性成纤维细胞生长因子;N-甲基-N-亚硝脲;细胞凋亡
Abstract： 目的研究非促分裂haFGF(nm-haFGF)对N-甲基-N-亚硝脲(MNU)所致大鼠视网膜损伤的保护作用及其作用机制.方法通过iP MNU(60mg·kg-1)复制大鼠视网膜损伤模型,0和12 h后,玻璃体腔内注射不同剂量nm-haFGF.24h和7d后,测量周边视网膜总厚度和外视网膜厚度、光感受器细胞凋亡指数及视网膜Bcl-2和Bax蛋白表达水平.结果 MNU作用24h后,nm-haFGF组周边视网膜的凋亡指数显著降低(P＜0.01);7d后,nm-haFGF组周边视网膜光感受器细胞数明显增多,且周边视网膜总厚度和外视网膜厚度增加(P＜0.01);不同剂量nm-haFGF可调节Bcl-2和Bax蛋白表达水平.结论 nm-haFGF能部分抑制MNU引起的SD大鼠视网膜损伤,其作用机制可能与上调Bcl-2和下调Bax蛋白表达水平有关.
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