Effects of Gingko biloba extract on glutamate-induced [ Ca2+ ]i changes in cultured cortical astrocytes after hypoxia/reoxygenation, H2O2 or L-glutamate injury
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 3
- DOI:
- 10.3321/j.issn:0513-4870.2005.03.005
- Key Word:
- 银杏叶提取物;星形胶质细胞;[Ca2+]i;低氧复氧;谷氨酸;H2O2;Gingko biloba extract;astrocyte;[ Ca2+ ]i;hypoxia/reoxygenation;glutamate;H2O2
Abstract： Aim To investigate glutamate-induced [ Ca2 + ] i changes in cultured rat neonatal cortical astrocytes after hypoxia/reoxygenation, H2O2 or high concentration of L-glutamate injury. In the meantime, the effects of Gingko biloba extract (GbE) were examined. Methods [ Ca2+ ]i changes in astrocytes were monitored by laser scanning confocal microscopy with the Ca2+ sensitive fluorescent probe [ Ca2 + ] i, but decrease by ( 3.3 ± 1.6) %, (81 ± 11 ) % and ( 81 ± 7 ) %, respectively. Pretreatment with H2O2 or high concentration of L-glutamate injury were ( 135 ± 98 ) %, ( 117 ± 93 ) % and ( 89 ± 36) %,different injury. Conclusion Hypoxia/reoxygenation, H2O2 and high concentration of L-glutamate impaired astrocytes' response to exogenous L-glutamate, and then bidirectional communication between astrocytes and neurons could not take place. GbE could improve the abnormal responses and maintain the normal function of astroglical network. These effects support that GbE has potential beneficial actions against brain injury.
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