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Iptakalim enhances astrocytic glutamate uptake activity
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 12
- DOI:
- 10.3321/j.issn:0513-4870.2004.12.005
- Key Word:
- 埃他卡林;6-羟基多巴胺;星形胶质细胞;谷氨酸摄取
Abstract： 目的研究新型ATP敏感性钾通道开放剂(KATPCO)埃他卡林(iptakalim,Ipt)对星形胶质细胞摄取谷氨酸的影响.方法取新生大鼠脑星形胶质细胞作原代培养,将Ipt直接作用于细胞,观察它对正常和6-羟基多巴胺(6-OHDA)损伤模型细胞摄取谷氨酸的影响;分别加入阳性对照药吡那地尔和非特异性钾通道阻断药格列本脲分析Ipt的作用机制.根据[3H]标记的D,L-谷氨酸摄入量判断细胞谷氨酸摄取作用强度.结果Ipt和吡那地尔都能增强星形胶质细胞的谷氨酸摄取作用、逆转6-OHDA引起的谷氨酸摄取抑制效应,预先加入格列本脲后,上述作用均被取消.结论埃他卡林可能通过促进钾通道开放增强星形胶质细胞摄取谷氨酸的作用.
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