Protective effect of M3 receptor on H2O2-induced apoptosis of rat myocardial cells in vitro
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 11
- DOI:
- 10.3321/j.issn:0513-4870.2004.11.005
- Key Word:
- M3受体;细胞凋亡;培养的心肌细胞;过氧化氢;原位缺口末端标记;共聚焦显微镜;钙;M3 receptor;apoptosis;cultured myocyte;hydrogen peroxide;in situ nick-end labeling;confocal microscope;calcium
Abstract： Aim To observe the effect of activation of Ms receptor on H2O2 induced apoptosis in cultured rat myocytes and to investigate its possible mechanisms. Methods Isolated neonatal cardiomyocytes were cultured. Morphologic changes were observed by microscopy. The apoptosis in cardiomyocyte was detected by terminal deoxynucleotide transferase directed d-UTP nick and end labeling (TUNEL) assay. The expression of apoptosis-related protein in Bcl-2 and Fas was measured by immunohistochemistry assay. [ Ca2+ ]i in single cardiomyocyte loaded with Fluo 3-AM was measured by confocal microscope. Results H2 O2-mediated tnyocyte apoptosis was attenuated by M3 receptor agonist Fas expression, and inhibited the increase in FI value of [ Ca2+ ]i in H2O2-stimulated cardiac myocytes.on H2O2 -stimulated cardiac myocytes. Conclusion Activation of M3 receptor showed protective effect on H2O2-induced apoptosis in cultured rat myocytes and this effect might be related to modulating the expression of some genes including Bcl-2 and Fas as well as the downregulation of [ Ca2 + ] i.
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