You Position: Home > Paper
Isolation and structure identification of grape seed polyphenols and its effects on oxidative damage to cellular DNA
( views:325, downloads:18 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 11
- DOI:
- 10.3321/j.issn:0513-4870.2004.11.002
- Key Word:
- 葡萄籽;抗氧化;葡酚酮;单细胞凝胶电泳
Abstract： 目的从葡萄籽中分离多酚化合物,进行抗氧化作用研究.方法采用Diaion HP-20,Toyopearl HW40柱色谱与制备型RP-HPLC相结合从葡萄籽乙酸乙酯萃取部位分离多酚物质,根据IR,MS,NMR,CD,X-射线单晶衍射等谱学数据并与已知化合物对照,确定化合物结构.对不同类型化合物经还原能力、DPPH·(α,α-diphenyl-β-picrylhydrazyl)自由基捕获试验进行抗氧化能力筛选,选择有抗氧化潜力的化合物施于小鼠脾细胞H2O2损伤模型,用单细胞凝胶电泳(SCGE,single cell gel-electrophoresis)技术分析化合物对细胞内DNA氧化损伤的防护作用.结果共分离到11个化合物(包括3个新结构),葡酚酮A,B,C,抗氧化试验表明,原花青素B4、儿茶素、表儿茶素与没食子酸的体外抗氧化能力较强,且低浓度(10 μmol·L-1,25 μmol·L-1)均表现对细胞DNA氧化损伤的防护作用,但150μmol·L-1儿茶素导致细胞DNA氧化损伤.结论葡酚酮A,B,C为首次报道.多酚类物质在低浓度有较好的细胞DNA氧化损伤的防护作用,可解释多酚物质的营养保健作用,而高浓度的多酚物质也可成为致氧化损伤物质,提示天然抗氧化剂的保健作用可能受剂量的影响.
- [1]魏雪涛,蒋建军,吴涛,等.姜黄素拮抗亚砷酸钠所致的DNA链断裂损伤[J].2002,(11).
- [2]Bagchi M,Williams C,Ye XM,etc.Acute and chronic stress-induced oxidative gastrointestinal injury in rats, and the protective ability of a novel grape seed proanthocyanidin extract.[J].1999,19(8).
- [3]Jorge M. Ricardo da Silva,Jacques Rigaud,Véronique Cheynier,etc.Procyanidin dimers and trimers from grape seeds[J].1991,30(4).
- [4]Corinne Prieur,Jacques Rigaud,Veronique Cheynier,etc.Oligomeric and polymeric procyanidins from grape seeds[J].1994,36(3).
- [5]P J, McCarthy,S F, Sweetman,P G, McKenna,etc.Evaluation of manual and image analysis quantification of DNA damage in the alkaline comet assay.[J].1997,12(4).
- [6]Das DK,Bagchi D,Maulik G,etc.Cardioprotective effects of grape seed proanthocyanidin against ischemic reperfusion injury.[J].1999,31(6).
- [7]E.N Frankel,J.B German,J.E Kinsella,etc.Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine[J].1993,341(8843).
- [8]Daneel Ferreira.Oligomeric proanthocyanidins: naturally occurring O-heterocycles[J].1996,13(5).
- [9]Peng HuiHsuan,Chen HuiYin,Yen GowChin.Antioxidant and pro-oxidant effects of various tea extracts.[J].1997,45(1).
- [10]Saint Cricq-De-Gaulejac-N,Provost C,Vivas N.Comparative study of polyphenol scavenging activities assessed by different methods.[J].1999,47(2).
- [11]Collins AR,Duthie SJ,Dobson VL,etc.Quercetin and myricetin protect against hydrogen peroxide-induced DNA damage (strand breaks and oxidised pyrimidines) in human lymphocytes.[J].1997,393(3).
- [12]Ohshima,H,Yoshie,Y,Auriol,S,etc.Antioxidant and pro-oxidant actions of flavonoids: effects on DNA damage induced by nitric oxide, peroxynitrite and nitroxyl anion.[J].1998,25(9).
- [13]S C, Sahu,M C, Washington.Effects of antioxidants on quercetin-induced nuclear DNA damage and lipid peroxidation.[J].1991,60(3).
- [14]Ray SD,Bagchi M,Bagchi D,etc.Free radicals and grape seed proanthocyanidin extract: importance in human health and disease prevention.[J].2000,148(2).
- [15]Duthie SJ,Dobson VL,Johnson W.The effect of dietary flavonoids on DNA damage (strand breaks and oxidised pyrimdines) and growth in human cells.[J].1997,390(1).
- [16]Waghorn G,Ulyatt MJ,Lu Y,etc.Proanthocyanidins from Lotus pedunculatus.[J].1997,45(8).
- [17]N P, Singh,M T, McCoy,R R, Tice,etc.A simple technique for quantitation of low levels of DNA damage in individual cells.[J].1988,175(1).