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Effects of montelukast on apoptosis and Fas mRNA expression of eosinophils in airway of asthmatic guinea pigs
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 10
- DOI:
- 10.3321/j.issn:0513-4870.2004.10.001
- Key Word:
- 白三烯受体拮抗剂;孟鲁司特;哮喘;嗜酸性粒细胞;细胞凋亡
Abstract： 目的研究白三烯受体拮抗剂孟鲁司特(montelukast,MK)对哮喘豚鼠气道嗜酸性粒细胞(eosinophil,Eos)凋亡和Fas mRNA表达的影响.方法以卵白蛋白致敏豚鼠制备哮喘模型.用密度梯度离心法分离并计数支气管肺泡灌洗液(BALF)中的嗜酸性粒细胞;采用TUNEL技术原位检测嗜酸性粒细胞凋亡;通过逆转录-多聚酶链反应(RT-PCR)技术检测嗜酸性粒细胞Fas mRNA的表达.结果孟鲁司特能显著降低哮喘豚鼠BALF中Eos的数量;在孟鲁司特治疗组,嗜酸性粒细胞凋亡指数明显升高,Fas mRNA的表达显著增强,与模型组比较均有显著性差异.结论嗜酸性粒细胞凋亡与Fas mRNA表达增加高度相关;增强气道嗜酸性粒细胞Fas mRNA的表达,促进其凋亡,可能是孟鲁司特拮抗哮喘气道炎症的一个重要机制.
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