You Position: Home > Paper
Effect of 17β-estradiol on Kv2.1 current and delayed rectifier potassium current in cultured rat hippocampal neurons
( views:191, downloads:5 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 9
- DOI:
- 10.3321/j.issn:0513-4870.2004.09.004
- Key Word:
- 17β-雌二醇;延迟整流钾电流;Kv2.1钾通道;HEK293细胞;海马;神经元;膜片钳技术
Abstract： 目的研究17β-雌二醇对Kv2.1钾电流和原代培养大鼠海马神经元延迟整流钾电流的作用.方法采用HEK293-Ky2.1细胞培养、大鼠海马神经元原代培养和膜片钳全细胞记录技术.结果17β-雌二醇浓度依赖地抑制Ky2.1钾电流和原代培养大鼠海马神经元延迟整流钾电流(IK).17β-雌二醇抑制Ky2.1钾电流和原代培养大鼠海马神经元延迟整流钾电流的IC50分别为2.4和4.0 μmol·L-1.通道动力学研究发现,17β-雌二醇(3 μmol·L-1)显著左移Ky2.1钾电流的稳态激活和失活曲线,但只显著左移海马神经元延迟整流钾电流稳态激活曲线,而不影响该电流的稳态失活曲线.结论17β-雌二醇抑制Ky2.1钾电流与抑制IK的程度相近,17β-雌二醇抑制IK的作用可能部分通过阻断Ky2.1钾电流而实现.
- [1]金宏伟,张炜,王晓良.慢性孵育β淀粉样肽25-35对培养大鼠海马神经元电压依赖性外向钾通道亚型mRNA表达的影响[J].2002,(8).
- [2]Harms C,Lautenschlager M,Bergk A,etc.Differential mechanisms of neuroprotection by 17 beta-estradiol in apoptotic versus necrotic neurodegeneration.[J].2001,21(8).
- [3]Y H, Bae,J Y, Hwang,Y H, Kim,etc.Anti-oxidative neuroprotection by estrogens in mouse cortical cultures.[J].2000,15(3).
- [4]L M, Mudd,J, Torres,T F, Lopez,etc.Effects of growth factors and estrogen on the development of septal cholinergic neurons from the rat.[J].1998,45(2).
- [5]M, Tamatani,S, Ogawa,G, Nu?ez,etc.Growth factors prevent changes in Bcl-2 and Bax expression and neuronal apoptosis induced by nitric oxide.[J].1998,5(10).
- [6]D.E. Antonucci,S.T. Lim,S. Vassanelli,etc.Dynamic localization and clustering of dendritic Kv2.1 voltage-dependent potassium channels in developing hippocampal neurons[J].2001,108(1).
- [7]S P, Yu,C H, Yeh,S L, Sensi,etc.Mediation of neuronal apoptosis by enhancement of outward potassium current.[J].1997,278(5335).
- [8]Trimmer JS,Murakoshi H.Identification of the Kv2.1 K+ channel as a major component of the delayed rectifier K+ current in rat hippocampal neurons.[J].1999,19(5).
- [9]DeFazio RA,Moenter SM.Estradiol feedback alters potassium currents and firing properties of gonadotropin-releasing hormone neurons.[J].2002,16(10).
- [10]S P, Yu,Z S, Farhangrazi,H S, Ying,etc.Enhancement of outward potassium current may participate in beta-amyloid peptide-induced cortical neuronal death.[J].1998,5(2).
- [11]Kim J,Montoya M,Hsieh D,etc.The women's health initiative estrogen replacement therapy is neurotrophic and neuroprotective.[J].2000,21(3).
- [12]Lim ST,Antonucci DE,Trimmer JS,etc.A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons.[J].2000,25(2).
- [13]Choi DW,Wang X,Gottron F,etc.Role of the outward delayed rectifier K+ current in ceramide-induced caspase activation and apoptosis in cultured cortical neurons.[J].1999,73(3).