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Effects of cyclovirobuxine D on intracellular Ca2+ and L-type Ca2+ current in rat ventricular cardiomyocytes
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 7
- DOI:
- 10.3321/j.issn:0513-4870.2004.07.005
- Key Word:
- 环维黄杨星D;心肌细胞;L型钙电流;细胞内Ca2+
Abstract： 目的研究环维黄杨星D(CD)对大鼠心室肌细胞内Ca2+动员和L型钙电流(ICa-L)的影响.方法采用全细胞膜片钳和激光扫描共聚焦显微术研究CD对心肌细胞ICa-L以及氯化钾、咖啡因诱发心肌细胞内Ca2+动员的影响.结果 CD浓度依赖性抑制ICa-L.指令电压为10 mV时,1和10μmol·L-1CD分别使ICa-L电流密度从(-9.9±1.8)pA/pF降至(-6.4±1.4)pA/pF和(-4.2±0.6)pA/pF.共聚焦实验显示1和10μmol·L-1CD不影响静息心肌细胞[Ca2+];,对氯化钾诱发[Ca2+]i升高水平无明显抑制作用;咖啡因引起的细胞内Ca2+动员可被CD进一步增强.结论 CD浓度依赖性抑制大鼠心室肌细胞Ica-L,并有促进咖啡因诱发心肌细胞内Ca2+释放的作用.
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