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Effects of puerarin on renal function,expressions of MMP-2 and TIMP-2 in diabetic rats
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 7
- DOI:
- 10.3321/j.issn:0513-4870.2004.07.001
- Key Word:
- 葛根素;糖尿病肾病;基质金属蛋白酶-2;基质金属蛋白酶组织抑制剂-2
Abstract： 目的探讨葛根素对糖尿病大鼠肾功能及肾组织基质金属蛋白酶2(MMP-2)及其组织抑制剂2(TIMP-2)表达的影响.方法单侧肾切除大鼠ip链脲佐菌素诱发糖尿病模型.用原位杂交法检测肾小球MMP-2及TIMP-2mRNA表达,流式细胞术和免疫组织化学检测肾皮质TGFβ1,MMP-2,TIMP-2,Ⅳ型胶原及层粘连蛋白表达.结果糖尿病组较对照组肾小球MMP-2 mRNA及蛋白表达降低而TIMP-2 mRNA及蛋白表达升高,TGFβ1,IV型胶原及层粘连蛋白表达亦增加,肾功能恶化;葛根素用药组较糖尿病组肾小球MMP-2 mRNA及蛋白表达升高,而TGFβ1,TIMP-2,Ⅳ型胶原及层粘连蛋白表达减少,肾功能改善.结论葛根素对糖尿病大鼠肾功能具有保护作用,除降低血糖外,调节肾小球MMP-2及TIMP-2表达,从而减轻肾小球细胞外基质沉积也可能是其作用途径之一.
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