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5-HT2c receptor subtype modulate production of secretory β-amyloid protein precursor in incubated rat hippocampal slices
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 5
- DOI:
- 10.3321/j.issn:0513-4870.2004.05.001
- Key Word:
- 分泌型淀粉样前体蛋白;海马;五羟色胺2C受体
Abstract： 目的考察5-HT2c受体对孵育海马脑片释放分泌型淀粉样前体蛋白(sAPP)的调节.方法应用5-HT、特异性5-HT2c受体激动剂M-110及其特异性拈抗剂L-107孵育海马脑薄片,及Western blot技术和特异性的针对sAPP氨基末端的单克隆抗体22C11检测释放到孵育液中的sAPP.结果 5-HT及特异性5-HT2c受体激动剂M-110在一定的浓度范围内呈浓度依赖性地显著增加sAPP分泌;而特异性5-HT2c受体拮抗剂L-107在一定的浓度范围内则浓度依赖性地显著抑制sAPP分泌.结论 5-HT通过激活5-HT2c受体调节孵育海马脑片分泌型淀粉样前体蛋白的释放.
- [1]G L, Caporaso,S E, Gandy,J D, Buxbaum,etc.Protein phosphorylation regulates secretion of Alzheimer beta/A4 amyloid precursor protein.[J].1992,89.
- [2]Ludmil Kirazov,Thomas Löffler,Reinhard Schliebs,etc.Glutamate-stimulated secretion of amyloid precursor protein from cortical rat brain slices[J].1997,30(6).
- [3]Govoni S,Trabucchi M,Gasparini L,etc.Specific role for protein kinase C alpha in the constitutive and regulated secretion of amyloid precursor protein in human skin fibroblasts.[J].1998,240(2).
- [4]Weber GA,Gendelman HE,Zheng J,etc.C1q-calreticulin induced oxidative neurotoxicity: relevance for the neuropathogenesis of Alzheimer's disease.[J].2003,135(1).
- [5]J D, Buxbaum,S E, Gandy,P, Cicchetti,etc.Processing of Alzheimer beta/A4 amyloid precursor protein: modulation by agents that regulate protein phosphorylation.[J].1990,87(15).
- [6]Nitsch RM,Deng M,Wurtman RJ,etc.Serotonin 5-HT2a and 5-HT2c receptors stimulate amyloid precursor protein ectodomain secretion.[J].1996,271(8).
- [7]P K, Smith,R I, Krohn,G T, Hermanson,etc.Measurement of protein using bicinchoninic acid.[J].1985,150(1).