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Inhibition of voltage-gated K+ current in rat intrapulmonary arterial smooth muscle cells by endothelin-1
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 1
- DOI:
- 10.3321/j.issn:0513-4870.2004.01.003
- Key Word:
- 钾通道;内皮素;膜片钳;肺动脉平滑肌细胞
Abstract： 目的观察内皮素(ET-1)对大鼠肺动脉平滑肌细胞(PASMCs)膜电压门控钾通道(KV)的作用.方法用全细胞膜片钳记录方法研究ET-1对膜电位(Em)、膜电容(Cm)、电压门控钾电流(IKV)的影响.结果 ET-1可引起PASMCs去极化,并抑制IKV,抑制IKV时间明显早于其对Em变化的影响,ET-1对IKV的影响呈可逆性和浓度依赖性,在有钙的环境中,ET-1对IKV峰电流的抑制率明显高于无钙时.结论 ET-1可浓度依赖性的抑制IKV,使常氧大鼠PASMCs去极化,且ET-1对IKV的抑制作用早于对Em的影响,这些作用不完全依赖于钙的参与,但钙可加强ET-1对IKV的抑制作用.
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