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Effects of 3-substituted aryl oxindole(PH II-7) on cell cycle of tumor cells
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 11
- DOI:
- 10.3321/j.issn:0513-4870.2003.11.002
- Key Word:
- 氧化吲哚衍生物;细胞周期;多药耐药;肿瘤细胞株
Abstract： 目的观察3-取代芳基氧化吲哚(PH II-7)对肿瘤细胞周期分布的影响,明确PH II-7的抗敏感肿瘤和耐药肿瘤的共同机制.方法用流式细胞仪检测细胞周期分布,Western印迹分析细胞周期相关蛋白的表达,3H-TdR参入法检测细胞DNA合成,ELISA测定酪氨酸激酶的活性.结果 PH II-7对多种肿瘤细胞(包括耐药细胞)的周期分布均有影响,阻滞细胞G1期至S期的移行,细胞周期相关蛋白CDK2,Rb和c-myc的表达被抑制,Cyclin E的表达升高.PH II-7还可抑制3H-TdR的参入,抑制EGFR的酪氨酸激酶的活性.结论抗耐药肿瘤新药PH II-7是一种细胞周期阻滞剂,可能是通过抑制CDK2而使肿瘤细胞阻滞在G1期,同时也说明细胞周期阻滞可能是抗耐药肿瘤的新方向.
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