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Inhibitory effect of egg white lysozyme on ceftazidime-induced release of endotoxin from Pseudomonas aeruginosa
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 11
- DOI:
- 10.3321/j.issn:0513-4870.2003.11.001
- Key Word:
- 鸡蛋清溶菌酶;内毒素;肿瘤坏死因子;抗生素
Abstract： 目的研究鸡蛋清溶菌酶(lysozyme, LZM)对头孢他啶(ceftazidime, CFT)诱导的铜绿假单孢菌内毒素释放的影响.方法在肉汤或稀释血液培养的铜绿假单孢菌液中加入LZM、CFT或LZM/CFT,作用一定时间后,测定培养上清液中的内毒素浓度;将细菌培养上清液加入到巨噬细胞RAW 264.7中或注射入小鼠体内,测定其炎性因子(NO和TNF-α)诱生能力.结果在肉汤培养和血液培养中,CFT引起铜绿假单孢菌迅速溶解和释放大量内毒素到培养上清液中,其上清液在体外培养的巨噬细胞上和小鼠体内可诱导大量NO和TNF-α产生.而LZM与CFT联合使用能阻止细菌溶解,降低细菌内毒素释放,减少炎性因子NO和TNF-α的产生.结论 LZM与CFT联合使用能抑制内毒素大量释放,减轻内毒素血症.
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