You Position: Home > Paper
Evodiamine induces A375-S2 cell death through two different pathways
( views:159, downloads:9 )
- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 9
- DOI:
- 10.3321/j.issn:0513-4870.2003.09.003
- Key Word:
- 吴茱萸碱;人黑色素瘤A375-S2;凋亡;抗肿瘤活性
Abstract： 目的研究吴茱萸碱诱导A375-S2细胞死亡机制.方法 MTT法测定吴茱萸碱对A375-S2的细胞毒作用.通过倒置光显微镜,荧光染色, DNA电泳观察细胞形态学变化.应用流式细胞分析技术研究药物对细胞周期的影响.结果吴茱萸碱明显抑制A375-S2生长,在24 h前可诱导A375-S2凋亡,亚二倍体峰出现,caspase蛋白酶被激活, 24 h后启动坏死途径,caspase蛋白酶抑制剂不能抑制细胞死亡.结论吴茱萸碱诱导A375-S2死亡时早期启动了caspase依赖性的非经典凋亡途径,后期则启动了坏死途径.
- [1]费晓方,王本祥,池岛乔.吴茱萸碱诱导人子宫颈癌HeLa细胞凋亡的机制研究[J].2002,(9).
- [2]Haddad EK,Henkart PA,Sarin A.Caspase dependence of target cell damage induced by cytotoxic lymphocytes.[J].1998,161(6).
- [3]G S, Salvesen,V M, Dixit.Caspases: intracellular signaling by proteolysis.[J].1997,91(4).
- [4]Wen-Fei Chiou,Cheng-Jen Chou,Andrew Yau-Chik Shum,etc.The vasorelaxant effect of evodiamine in rat isolated mesenteric arteries: mode of action[J].1992,215(2-3).
- [5]Hiroko Tada,Osamu Shiho,Ken-ichi Kuroshima,etc.An improved colorimetric assay for interleukin 2[J].1986,93(2).
- [6]M A, Hotz,G, Del Bino,P, Lassota,etc.Cytostatic and cytotoxic effects of fostriecin on human promyelocytic HL-60 and lymphocytic MOLT-4 leukemic cells.[J].1992,52.
- [7]Naokatu Arakaki,Jamil Ahsan Kazi,Takehiro Kazihara,etc.Hepatocyte Growth Factor/Scatter Factor Activates the Apoptosis Signaling Pathway by Increasing Caspase-3 Activity in Sarcoma 180 Cells[J].1998,245(1).
- [8]Apurva Sarin,Jishy Vaarhese,Souvik Chattopadhaya.Inhibition of p38 Kinase reveals a TNF-#alpha[J].2001,166(11).
- [9]Masaru Ogasawara,Toshiyuki Matsubara,Hideyo Suzuki.Inhibitory Effects of Evodiamine on in Vitro Invasion and Experimental Lung Metastasis of Murine Colon Cancer Cells[J].2001,24(8).
- [10]Daniel R. Catchpoole,Bernard W. Stewart.Formation of Apoptotic Bodies Is Associated with Internucleosomal DNA Fragmentation during Drug-Induced Apoptosis[J].1995,216(1).