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Investigations on the molecular mechanisms of saponins from Anemarrhena asphodeloides Bunge using oligonucleotide microarrays
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- Author:
- No author available
- Journal Title:
- ACTA PHARMACEUTICA SINICA
- Issue:
- 7
- DOI:
- 10.3321/j.issn:0513-4870.2003.07.006
- Key Word:
- 知母;皂苷;基因芯片;基因表达
Abstract： 目的研究知母皂苷活血化瘀作用的分子机制.方法制备了包含87个心血管疾病相关基因探针的寡核苷酸芯片.将80 mg*L-1 知母皂苷作用8 h及未作用的培养人血管内皮细胞提取总RNA,通过逆转录分别标记Cy 5和Cy 3荧光染料,然后与芯片杂交,检测知母皂苷对血管内皮细胞中心血管疾病相关基因表达的影响. 结果知母皂苷作用于血管内皮细胞后,血管紧张素酶原(AGT)基因、肾上腺素α2A受体(ADRA2R)基因和内皮素转换酶-1(ECE-1)基因的表达均有不同程度的下调.结论知母皂苷有调控血管内皮细胞功能的作用,可能是其治疗心血管疾病的机制之一.
- [1]黄坚,陈苏红,佟丽,等.小鼠细胞因子相关基因表达检测寡核苷酸芯片的制备及分析[J].2002,(4).
- [2]陈忠斌,杨静,王升启.cDNA芯片阳性对照的制备及在芯片敏感性分析中的应用[J].2002,(5).
- [3]C R, Bacon,N R, Cary,A P, Davenport.Endothelin peptide and receptors in human atherosclerotic coronary artery and aorta.[J].1996,79(4).
- [4]E.M.Southern,GreenCase,J.K.EIder,etc.Arrays of complementary oligonucleotides for analysing the hybridisation behaviour of nucleic acids[J].1994,22(8).
- [5]J L, Zhuo,P, Froomes,D, Casley,etc.Perindopril chronically inhibits angiotensin-converting enzyme in both the endothelium and adventitia of the internal mammary artery in patients with ischemic heart disease.[J].1997,96(1).
- [6]J N, Topper,J, Cai,D, Falb,etc.Identification of vascular endothelial genes differentially responsive to fluid mechanical stimuli: cyclooxygenase-2, manganese superoxide dismutase, and endothelial cell nitric oxide synthase are selectively up-regulated by steady laminar shear stress.[J].1996,93(19).
- [7]Dzau VJ.,Horiuchi M.,Hayashida W.,etc.The Angiotensin II Type 2 (at(2)) Receptor Antagonizes the Growth Effects of the at(1) Receptor - Gain-of-Function Study Using Gene Transfer[J].1995,92(23).
- [8]Noboru Nakashima,Ikuko Kimura,Masayasu Kimura,etc.Isolation of Pseudoprototimosaponin AIII from Rhizomes of Anemarrhena asphodeloides and Its Hypoglycemic Activity in Streptozotocin-Induced Diabetic Mice[J].1993,56(3).
- [9]Nobuo Nyui,Kouichi Tamura,Satoshi Yamaguchi,etc.Tissue Angiotensinogen Gene Expression Induced by Lipopolysaccharide in Hypertensive Rats[J].1997,30(4).
- [10]Ader P,Maguire JJ,Gohil K,etc.The in vivo neuromodulatory effects of the herbal medicine ginkgo biloba.[J].2001,98(12).
- [11]Schena, Mark,Shalon, Dari.Quantitative monitoring of gene expression patterns with a complimentary DNA microarray.[J].1995,270(5235).
- [12]J N, Cohn.Is there a role for endothelin in the natural history of heart failure?[J].1996,94(4).